InP/ZnS QDs对稀有鮈鲫子代软骨发育的影响

Effects of InP/ZnS QDs on cartilage development in rare minnow(Gobiocypris rarus)offspring

使用InP/ZnS QDs以雌性稀有鮈鲫(Gobiocypris rarus)为实验动物,通过腹腔注射染毒,设计了(200,400,800nmol/L)3个实验组,在量子点暴露4和7d时取卵受精.以胚胎受精率、存活率、仔鱼体长、全长为指标,对仔鱼进行阿利新蓝染色和HE(Hematoxylin Eosin)染色,通过检测发育相关基因(bmp2b,sox9a,runx_(2)b)的相对表达量,研究InP/ZnS QDs对稀有鮈鲫子代软骨发育的影响.结果表明:高浓度组仔鱼的体长减少6.2%、全长减少5.9%;颅面PQ-Meckel角增加24.8%,下颌骨长度减小14.6%和15.2%、宽度减小10.0%和10.7%;颅面软骨细胞肿大、数量减少.并发现QDs在不同发育时间对相关基因的相对表达量影响不同.总之InP/ZnS QDs会对稀有鮈鲫子代软骨发育产生不良影响.

Quantum Dots(QDs)are a class of nanomaterials.With the wide application of QDs,its toxic affects on organisms are also concerned.In this experiment,InP/ZnS quantum dots were used,and female rare minnows(Gobiocypris rarus)was used as the experimental animal.Three experimental groups(200,400,800nmol/L)were designed.Eggs were taken and fertilized at 4 and 7 days of quantum dots exposure.Using embryo fertilization rate,survival rate,body length and full length of larvae as indicators,the larvae were observed by Albion blue dye and Hematoxylin Eosin(HE)staining.The transcript expression levels of bone developmental related genes(bmp2b,sox9,runx_(2)b)were detected to study the effect of quantum dots on the offspring cartilage development of rare minnows.The results showed that the body length and full length of larvae in high concentration group decreased by 6.2%and 5.9%.The PQ-Meckel’s angle increased by 24.8%,mandibular length decreased by 14.6%and 15.2%,and mandibular width decreased by 10.0%and 10.7%.Craniofacial chondrocytes were swelled and reduced in number.It was found that QDs had different affects on the transcript expression levels of related genes at different development time.In conclusion,InP/ZnS quantum dots can affect the skeletal development of offspring of rare minnows.