超声引导下鼠肝消融术后组织病理特征的变化规律及影响

Patterns and effects of changes in histopathological characteristics after ultrasound-guided murine liver ablation

目的探讨超声引导下鼠肝微波消融术(MWA)后近期消融区内组织病理特征随时间的变化规律及影响。方法建立SD大鼠肝脏MWA模型,在超声引导下对SD大鼠的肝脏部位行MWA,根据术后不同时间,将SD大鼠随机分为MWA术后即刻组、术后2 h组、术后6 h组、术后12 h组,每组6只大鼠。观察各组SD大鼠的消融区体积和肝脏消融比、消融区肝脏组织的病理学形态、细胞凋亡情况及炎性细胞因子水平。结果超声评估显示,MWA术后消融区体积和肝脏消融比随时间增加而减小,术后时间和肝脏消融比存在相关性(r=-0.7714,P<0.0001)。HE染色显示,大鼠肝脏消融区组织随着MWA术后时间的增加而出现明显的坏死结构。TUNEL染色结果显示,大鼠肝脏消融区组织细胞的凋亡水平随着MWA术后时间的增加而增加,两者存在相关性(r=0.9645,P<0.0001)。大鼠肝脏消融区组织的炎性细胞因子IL-1β和IL-18水平随着MWA术后时间的增加而降低,IL-1β水平(r=-0.9192,P<0.0001)和IL-18水平(r=-0.8225,P<0.0001)均与MWA术后时间存在相关性。结论在超声引导下对SD大鼠肝脏部位行MWA术,术后时间与肝脏消融比、消融区组织的炎性细胞因子IL-1β和IL-18水平呈负相关,术后时间与消融区组织细胞凋亡率呈正相关。

Objective To investigate the changing law of histopathological features in the recently ablated area after ultrasound-guided microwave ablation(MWA)of rat liver with time and its influence.Methods A MWA model of the liver of SD rats was established,and MWA was performed on the liver sites of SD rats under ultrasound guidance.According to the different times after the operation,SD rats were randomly divided into immediately post-operative MWA,post-operative MWA group of 2 h,post-operative MWA group of 6 h,and post-operative MWA group of 12 h,with 6 rats in each group.The volume of ablation area and liver ablation ratio,pathological morphology of liver tissue in the ablation area,apoptosis and inflammatory cytokine levels were observed in SD rats in each group.Results Ultrasound evaluation showed that the volume of ablation area and liver ablation ratio decreased with the increase of time after MWA,and there was a correlation between the postoperative time and liver ablation ratio(r=-0.7714,P<0.0001).HE staining showed that the tissue of the ablation area of the rat liver showed obvious necrotic structures with the increase of the postoperative time after MWA.TUNEL staining showed that the apoptosis level of the tissue cells in the ablation area of rat liver increased with the increase of time after MWA,and there was a correlation between the two(r=0.9645,P<0.0001).The levels of inflammatory cytokines IL-1βand IL-18 in the tissues of the ablation area of rat liver decreased with the increase of the time after MWA,and there was a correlation between the levels of IL-1β(r=-0.9192,P<0.0001)and IL-18(r=-0.8225,P<0.0001)with the time after MWA.Conclusion After ultrasound-guided MWA on the liver of SD rats,there is a negative correlation between the postoperative time and the ablation ratio of the liver,and the levels of inflammatory cytokines IL-1βand IL-18 in the tissue of the ablation area,and a positive correlation between the postoperative time of the MWA and the rate of apoptosis in the tissue of the ablation area.