白星花金龟幼虫虫粪可培养微生物的分离鉴定及特性研究

Isolation,Identification and Characterization of Culturable Microorganisms from Frass of Protaetia brevitarsis Larva

[目的]筛选可培养功能微生物,为农业废弃物的循环利用提供微生物资源。[方法]以喂食废菌渣的白星花金龟幼虫虫粪为研究对象,采用平板涂布法分离虫粪中的可培养微生物,并对其分解纤维素和蛋白质能力进行初步探究。[结果]共分离出细菌5株、真菌3株,经鉴定,分别为纤维化纤维微细菌、铅黄肠球菌、原玻璃蝇节杆菌、谷氨酸杆菌、灰色链霉菌、热带假丝酵母、长枝木霉和白地霉。其中纤维化纤维微细菌和灰色链霉菌具有降解蛋白质的能力,在酪蛋白培养基上透明圈直径与菌落直径的比值(HC)分别为7.259±0.417和1.971±0.584;原玻璃蝇节杆菌、灰色链霉菌、白地霉、长枝木霉具有降解纤维素的能力,在羧甲基纤维素钠上经刚果红染色后透明圈HC分别为1.166±0.028、2.044±0.245、1.075±0.029、1.288±0.048。[结论]纤维化纤维微细菌、原玻璃蝇节杆菌、灰色链霉菌、白地霉和长枝木霉可作为食用菌废菌渣等农业废弃物循环利用的菌种资源。

[Objective]To screen cultivable functional microorganisms,and provide microbial resources for the recycling of agricultural waste.[Method]Taking the frass of Protaetia brevitarsis larva with the edible fungi residues as the research object,the plate coating method was used to separate culturable microorganisms from feces,and their ability to decompose cellulose and protein was preliminarily explored.[Result]A total of 5 strains of bacteria,and 3 strains of fungi were isolated.After identification,they were identified as Cellulosimicrobium cellulans,Enterococcus casseliflavus,Arthrobacter protophormiae,Glutamicibacter halophytocola,Streptomyces griseolus,Candida tropicalis,Trichoderma longibrachiatum,Geotrichum candidum.Among them,Cellulosimicrobium cellulans and Streptomyces griseolus had the ability to decompose protein,and the ratio of transparent circle diameter to colony diameter(HC)on casein culture medium were 7.259±0.417 and 1.971±0.584,respectively.Arthrobacter protophormiae,Streptomyces griseolus,Geotrichum candidum and Trichoderma longibrachiatum had the ability to degrade cellulose,and after Congo red staining on sodium carboxymethyl cellulose,the HC values of the transparent circle were 1.166±0.028,2.044±0.245,1.075±0.029 and 1.288±0.048.[Conclusion]Cellulosimicrobium cellulans,Arthrobacter protophormiae,Streptomyces griseolus,Geotrichum candidum and Trichoderma longibrachiatum can be used as strain resources for the reuse of agricultural wastes such as edible fungi residues.