鹌鹑MyoD基因多态性与生长性状的相关性分析

The Polymorphism Detection of MyoD Gene and Correlation Analysis with Growth Traits in Quail

[目的]研究MyoD基因与鹌鹑生长性状的相关性。[方法]以朝鲜鹌鹑为研究对象,应用HRM技术检测群体中MyoD基因的多态性,测定其生长性状,并进行关联分析;qPCR检测3种基因型胚胎肌肉组织中MyoD基因的表达量。[结果]MyoD基因在群体中存在AA、Aa和aa 3种基因型,AA和Aa基因型个体的胸围显著高于aa基因型(P<0.05)。胚胎发育的7~15 d均能检测到MyoD基因表达,MyoD基因的表达量从11 d开始升高,15 d达到峰值。AA和Aa基因型MyoD基因表达量提高速度高于aa基因型。[结论]MyoD基因A等位基因为朝鲜鹌鹑胚胎生长发育的优势基因,可用于生长发育快的个体基因型育种选择。

[Objective]The purpose of this study was to explore the correlation between the different genotypes of MyoD gene and growth traits in quails.[Method]Korean quails were chosen as the research object,HRM technology was used to detect the polymorphism of MyoD gene in quails,and the growth traits were measured.The correlation analysis between growth traits and MyoD gene were analyzed.The mRNA expression of MyoD three genotypes were analyzed by qPCR in muscle tissue in embryonic stage of quail.[Result]The results showed that three genotypes(AA,Aa and aa)of MyoD gene were detected in the quail population.The chest circumference was significantly higher in AA and Aa genotype than aa genotype(P<0.05).The results of qPCR showed that MyoD gene expression could be detected at 7-15 d of embryonic development,the level of MyoD gene expression was increased on day 11,and reached a peak on day 15 of embryonic development.The expression level of AA and Aa genotypes was significantly faster than that of aa genotype.[Conclusion]It is speculated that A allele of MyoD gene may be the dominant genotype during the embryonic growth and development of Korean quail,and can be used to genotype selection for rapid growth in quail breeding.