褶皱臂尾轮虫(Branchionus plicatilis)响应四溴联苯醚(BDE-47)胁迫的能量代谢变化研究

THE CHANGES OF ENERGY METABOLISM OF ROTIFER BRACHIONUS PLICATILIS IN RESPONSE TO BDE-47 STRESS

多溴联苯醚(PBDEs)作为一种典型的持久性有机污染物,对海洋生物和生态系统的可持续性构成严重威胁。以2,2’,4,4’-四溴联苯醚(BDE-47)作为胁迫因子,以褶皱臂尾轮虫(Branchionus plicatilis)作为受试生物,基于代谢组学技术探讨褶皱臂尾轮虫应对BDE-47胁迫时的能量代谢变化。24 h胁迫实验的结果表明:褶皱臂尾轮虫的滤水率(F)和摄食率(G)显著降低,抑制了其对能源物质的获取;褶皱臂尾轮虫α-淀粉酶、脂肪酶、胰蛋白酶活性均受到抑制,降低了其对能源物质的消化效率;褶皱臂尾轮虫的总腺苷酸含量(TAP)随着BDE-47胁迫浓度的增加而不断下降,其能量状态发生了不利变化;褶皱臂尾轮虫的细胞能量分配(CEA)降低,是能量储备(E_(a))减少和能量消耗(E_(c))增加共同作用的结果。代谢组学分析表明褶皱臂尾轮虫的氨基酸代谢、脂质代谢和糖代谢产生了显著变化,发现了与氨基酸代谢、脂质代谢和糖代谢相关的26种差异代谢物和20条代谢通路,氨基酸、脂肪酸以及果糖水平显著下调,溶血磷脂酰胆碱(lysoPC)和磷酸乙醇胺(PE)显著上调。以上结果表明,BDE-47急性毒性胁迫影响了褶皱臂尾轮虫对于能源物质的获取和利用,对褶皱臂尾轮虫的能量状态产生负面影响,并且使其在能量分配上发生了改变。

As typical persistent organic pollutants,polybrominated biphenyl ethers(PBDEs)pose a serious threat to the sustainability of marine life and ecosystems.Using Tetra-BDE(BDE-47)as the stress factor and rotifer Branchionus plicatilis as the test organism,the energy-related changes of B.plicatilis in response to BDE-47 stress were studied in metabonomics.The results of BDE-47 stress for 24 hours showed that:the filtering rate and the feeding rate of B.plicatilis decreased significantly,which inhibited its acquisition of energy materials.The activities ofα-amylase,lipase,and trypsin of B.plicatilis were all inhibited,which reduced its digestion efficiency of energy substances.The total adenylate content(TAP)of B.plicatilis decreased with the increase of BDE-47 concentration,and its energy state changed unfavorably.The decrease of cellular energy distribution of B.plicatilis is the result of the decrease of energy reserve and the increase of energy consumption.Metabonomic analysis showed that there were significant changes in amino acid metabolism,lipid metabolism,and glucose metabolism of B.plicatilis.Twenty-six differential metabolites and 20 metabolic pathways related to amino acid metabolism,lipid metabolism,and glucose metabolism were identified.Amino acid,fatty acid and fructose levels were down-regulated,and lysophosphatidylcholine and phosphoethanolamine were up-regulated.Therefore,the acute toxic stress of BDE-47 affected the acquisition and utilization of energy substances,had a negative effect on the energy state of B.plicatilis,and changed its energy distribution.