Fe^(3+)金属有机骨架载药纳米颗粒的制备及应用

Preparation and application of Fe^(3+) metal-organic frameworks drug-loaded nanoparticles

金属有机骨架(MOFs)具有高孔隙率和比表面积大特点,可以用作药物递送载体靶向治疗肿瘤。首先,通过水热法在马弗炉内高温结晶合成金属有机骨架纳米颗粒(MIL-88B-NH_(2))。负载精氨酸(D-Arg)和葡萄糖氧化酶(GOX)形成D-Arg@MIL-88B-NH_(2)/GOX纳米颗粒。采用透射电镜(TEM)、扫描电镜(SEM)、Zeta电位、红外光谱(FT-IR)和电感耦合等离子体发射光谱(ICP-AES)等手段对纳米颗粒进行表征。结果表明:所制备的纳米材料粒径均一(约为190nm),具有较为立体的梭形结构,同时通过表征证实了载药纳米颗粒的成功制备。体外细胞实验表明纳米颗粒可以靶向癌细胞、提高抗肿瘤效果和促进细胞凋亡。

Metal-organic frameworks(MOFs)can be used as a drug delivery carrier for targeted treatment of tumors due to their high porosity and large specific surface area.First,metal-organic framework nanoparticles(MIL-88B-NH 2)was synthesized by hydrothermal crystallization in a muffle furnace at high temperature.Then,D-Arg@MIL-88B-NH 2/GOX nanoparticles were formed by loading Arginine(D-Arg)and Glucose Oxidase(GOX).The nanoparticles were characterized by TEM,SEM,FT-IR,Zeta potentials and ICP-AES.The results showed that the prepared nanomaterials had a uniform particle size(about 190nm)and a relatively three-dimensional shuttle structure.At the same time,the successful preparation of drug-loaded nanoparticles was confirmed by characterization.In vitro cell experiments indicated that nanoparticles could target cancer cells,improve anti-tumor effect and promote cell apoptosis.