蔬菜膳食纤维基多菌群乳酸菌生物膜的制备、表征及其稳定性分析

Preparation,characterization and stability analysis of vegetable dietary fiber-based multi-flora lactic acid bacteria biofilm

试验采用自主设计的生物膜形成培养装置,以8种乳酸菌类益生菌为菌种,以MRS复合甘蓝、芹菜、黄瓜、冬瓜等4种蔬菜浆液为营养液,以营养液中的水不溶性蔬菜膳食纤维为载体,进行连续动态培养.结果表明,37℃培养7天时,形成的乳酸菌与蔬菜膳食纤维复合生物膜中的菌浓可达2.24×10^(12)CFU/g,显微表征和菌群结构分析结果表明,乳杆菌属和片球菌属均得到了富集.对获得的乳酸菌与蔬菜膳食纤维复合生物膜进行了贮存试验及模拟消化试验,结果显示25℃6个月后,活菌数仍在10^(6)CFU/g以上,模拟消化后活菌数保持在10^(12)CFU/g以上.本试验结果可为高菌浓益生菌发酵剂、益生菌包埋材料以及益生菌膳食补充剂等的开发提供理论依据及技术支撑.

In this study,a self-designed biofilm-forming culture device was used,with 8 lactic acid bacteria probiotics as strains,MRS was compounded with cabbage,celery,cucumber and wax gourd vegetable juice as nutrient solution,and water-insoluble vegetable dietary fiber in nutrient solution as carrier for continuous dynamic culture.The results showed that the bacterial concentration in the composite biofilm of lactic acid bacteria-vegetable dietary fiber could reach 2.24×10^(12) CFU/g after 7 days of incubation at 37℃.The results of microscopic characterization and flora structure analysis showed that both Lactobacillus and Pediococcus were enriched.The storage test and simulated digestion test of the obtained biofilms of lactic acid bacteria-vegetable dietary fiber were conducted,and the results showed that the viable count remained above 10^(6) CFU/g after 6 months at 25℃and above 10^(12) CFU/g after simulated digestion.The test results can provide theoretical basis and technical support for the development of high bacterial concentration probiotics starter cultures,probiotics embedding materials and probiotics dietary supplements.