酶标仪在艾滋病血液检验中的应用价值体会

Application value of microplate reader in acquired immune deficiency syndrome blood test

目的分析在艾滋病(AIDS)血液检验中酶标仪的应用价值。方法60例已经确诊为AIDS的患者,均进行酶标仪检验、血液分析仪检验。对比两种检验方法精密度,分析线性范围;对比两种检验方法标本回收率,分析稳定性。结果对3份血清进行批间、批内20次的重复检测,酶标仪检验的批内精密度、批间精密度均明显高于血液分析仪,差异具有统计学意义(P<0.05)。AIDS血清的线性范围临界值是160 KU,在AIDS活性达到上述标准后,可利用等渗盐水对其进行稀释,同时将检测工作完成。酶标仪检验的标本平均回收率是98.2%,高于血液分析仪的94.4%,差异具有统计学意义(P<0.05)。在一份AIDS血液标本添加氢氧化钠(NaOH),浓度为0.4 mmol/L,在显色后的不同时间实施比色,结果指出,10~90 min显色比较稳定,显色10 min后比色情况良好。结论通过酶标仪测定AIDS患者的血液存在快速、简便等优势,效果理想,成本低,时间短。

Objective To analyze the application value of microplate reader in acquired immune deficiency syndrome(AIDS)blood test.Methods 60 patients who had been diagnosed with AIDS were tested by microplate reader and blood analyzer.The precision of the two detection methods was compared,and the linear range was analyzed.The sample recovery rate of the two detection methods was compared,and the stability was analyzed.Results Three serum samples were repeatedly tested 20 times within and between batches.The intra batch precision and inter batch precision of the microplate reader were higher than those of the blood analyzer,and the differences were statistically significant(P<0.05).The linear range threshold for AIDS serum was 160 kU,and after the AIDS activity reached the above criteria,isotonic saline could be utilized to dilute it while the assay was being completed.The average recovery rate of the sample of the microplate reader was 98.2%,which was significantly higher than that of 94.4%of the blood analyzer,and the difference was statistically significant(P<0.05).Sodium hydroxide(NaOH)was added to AIDS serum samples at a concentration of 0.4 mmol/L,and colorimetry was carried out at different time points after color development.The results showed that the time of stable color development was 10-90 min,and the color comparison in color development was better after 10 min.Conclusion The blood of AIDS patients detected by microplate reader has the advantages of rapidity,simplicity,ideal effect,low cost and short time.