Effects of Homoharringtonine on the Proliferation and Apoptosis of Melanoma Cells

[Objectives]To explore the effects of homoharringtonine(HHT)on the proliferation and apoptosis of melanoma cells.[Methods]Mouse melanoma cell line B16F10 was divided into 5 dfferent dose groups,among which Oμg/mL HHT was as the negative blank group,and the remaining four groups were added with different concentrations of HHT(20,40,80 and i60μg/mL).After B16F10 cells were treated with HHT for 48 h,the growth inhibition rate of B16F10 cells was observed,and the cell cycle was detected.Cyclin D1,MMP-9 and VEGF mRNA and protein levels were detected by qRT-PCR and Western-blot.[Results]Compared with Oμg/mL group,after different concentrations of HHT treated B16F10 cells,the inhibition rate and apoptosis rate of B16F10 cells increased,and Cyclin D1,MMP-9 and VECF mRNA and protein levels in all groups decreased significantly,with statistical significance(P<0.05).[Conclusions]HHT may be involved in the apoptosis of melanoma cells by regulating Cyclin DI level,affecting cell cycle,and inhibiting VEGF and MMP-9 gene protein levels.