VEGFB基因敲除对动脉粥样硬化的作用研究

Effect of VEGFB gene knockout on atherosclerosis

目的为探究血管内皮生长因子B(VEGFB)基因在动脉粥样硬化中的作用,构建ApoE-/-/VEGFB-/-双敲小鼠模型进行研究。方法实验共分为4组:高脂饮食ApoE-/-/VEGFB-/-组(WD-ApoE-/-/VEGFB-/-)、普通饮食ApoE-/-/VEGFB-/-组(Chow-ApoE-/-/VEGFB-/-)、高脂饮食ApoE-/-组(WD-ApoE-/-)和普通饮食ApoE-/-组(Chow-ApoE-/-)。每周测定小鼠体重和血脂变化,对主动脉进行油红O染色,取心脏进行冰冻切片油红O及H-E染色,分析小鼠主动脉斑块沉积变化。结果WD-ApoE-/-/VEGFB-/-组体重、总胆固醇、甘油三酯、低密度脂蛋白显著高于WD-ApoE-/-组;主动脉大体油红O染色结果显示:相较于ApoE-/-小鼠,ApoE-/-/VEGFB-/-小鼠主动脉脂质斑块面积显著增加。冰冻切片油红O染色结果显示:相较于ApoE-/-小鼠,ApoE-/-/VEGFB-/-小鼠主动脉根部脂质斑块面积显著增加。冰冻切片H-E染色结果显示:相较于ApoE-/-小鼠,ApoE-/-/VEGFB-/-小鼠主动脉根部斑块坏死面积显著增加。结论VEGFB-/-可以通过增加主动脉脂质斑块沉积,增加斑块坏死面积,促进动脉粥样硬化的进展。

Objective To investigate the role of VEGFB gene in the progression of atherosclerosis through establishing an ApoE-/-/VEGFB-/-mouse model.Methods There were four groups in the current experiment:a high-lipid diet ApoE-/-/VEGFB-/-group(WD-ApoE-/-/VEGFB-/-),a common diet ApoE-/-/VEGFB-/-group(Chow-ApoE-/-/VEGFB-/-),a high-lipid diet ApoE-/-group(WD-ApoE-/-)and a common diet ApoE-/-group(Chow-ApoE-/-).The mice were weighed every week,and their changes in blood lipids were measured.The aortas were stained with oil Red O,and the hearts were collected,cut into frozen sections and stained with oil Red O and H-E to analyze the changes of plaque deposition in the aorta of the mice.Results The WD-ApoE-/-/VEGFB-/-group showed remarkable increases in body weight,total cholesterol,triglyceride,and low-density lipoprotein,compared with the WD-ApoE-/-group.According to the oil red O staining,increased lipid plaque areas were observed in the aorta of ApoE-/-/VEGFB-/-mice,compared with ApoE-/-mice.Frozen sections also indicated enlarged lipid plaque area in the aorta root of ApoE-/-/VEGFB-/-mice,compared with ApoE-/-mice.HE staining confirmed increased necrotic area in the aortic root of ApoE-/-/VEGFB-/-mice,compared with ApoE-/-mice.Conclusions VEGFB-/-can effectively increase the deposition of aortic lipid plaque,enlarge necrotic area,and promote the progression of atherosclerosis.