人参皂苷Rg3增加卵巢癌细胞对顺铂敏感性及机制研究

Ginsenoside Rg3 Increases the Sensitivity of Ovarian Cancer Cells to Cisplatin and Its Mechanism

目的:探究人参皂苷Rg3对卵巢癌细胞SKOV3的增殖及顺铂增敏功能的效果及其作用机制。方法:CCK-8法测人参皂苷Rg3和顺铂对卵巢癌细胞的细胞活度;倒置显微镜观察细胞形态;划痕实验探究细胞迁移;克隆形成实验探讨人参皂苷Rg3对SKOV3细胞克隆产生能力的作用;Westernblot实验探讨人参皂苷Rg3对SKOV3细胞PI3K/Akt/mTOR信号通路表达水平的效力。结果:人参皂苷Rg3对SKOV3细胞呈时间和剂量依赖性抑制,人参皂苷Rg3可增加SKOV3细胞对顺铂的敏感性。在镜下查看细胞,结果发现和单一使用DDP相比,加药后细胞变圆,凋亡细胞增多。克隆形成数据得出,顺铂的浓度抬升,SKOV3细胞的克隆形成数量显著削减,加入人参皂苷Rg3能进一步抑制;划痕实验实验结果可以显示,人参皂苷Rg3能够进一步抑制DDP作用下的卵巢癌细胞的迁移效果。Western blot探究数据看出,人参皂苷Rg3可使PI3K/Akt/mTOR信号通路蛋白表达量降低。结论:人参皂苷Rg3可控制SKOV3细胞的增殖和进展,可-1-www.ivypub.org/bf以促进细胞对顺铂的敏感性,对PI3K/Akt/mTOR信号通路表达具有抑制效果,并通过抑制该信号通路表达增加对顺铂的敏感性。

To investigate the effect and mechanism of ginsenoside Rg3 on proliferation and cisplatin sensitization of ovarian cancer cells SKOV3.Methods:The cell activity of ginsenoside Rg3 and cisplatin on ovarian cancer cells was measured by CCK-8 method.Cell morphology was observed by inverted microscope.Cell migration was investigated by scratch test.To investigate the effect of ginsenoside Rg3 on the cloning ability of SKOV3 cells.Western blot assay was used to investigate the effect of ginsenoside Rg3 on the expression level of PI3K/Akt/mTOR signaling pathway in SKOV3 cells.Results:Ginsenoside Rg3 inhibited SKOV3 cells in a time-dependent and dose-dependent manner.Ginsenoside Rg3 increased SKOV3 cells'sensitivity to cisplatin.When the cells were examined under microscope,it was found that compared with DDP alone,the cells became round and apoptotic cells increased.The clonal formation data showed that with the increase of cisplatin concentration,the number of clonal formation of SKOV3 cells was significantly reduced,and the addition of ginsenoside Rg3 could further inhibit the formation.Scratch experiments showed that ginsenoside Rg3 could further inhibit the migration of ovarian cancer cells under DDP.Western blot analysis showed that ginsenoside Rg3 could reduce the protein expression level of PI3K/Akt/mTOR signaling pathway.Conclusion:Ginsenoside Rg3 can control the proliferation and progression of SKOV3 cells,promote cell sensitivity to cisplatin,inhibit the expression of PI3K/Akt/mTOR signaling pathway,and increase the sensitivity to cisplatin by inhibiting the expression of this signaling pathway.