P16/Ki-67双染、TCT细胞学、HPV联合检测技术在宫颈病变筛查中的应用价值分析

Analysis of the Application Value of P16/Ki-67 Double Staining,TCT Cytology,and HPV Combined Detection Technology in Cervical Lesion Screening

目的探讨与分析P16/Ki-67双染、液基薄层细胞学检查(Thinprep Cytologic Test,TCT)、人乳头状瘤病毒(Human Papilloma Virus,HPV)联合检测技术在宫颈病变筛查中的应用价值。方法收集2022年4月—2023年4月选择在酒钢医院自愿行宫颈病变筛查且后期进行宫颈组织活检的83例就诊人群为研究对象,所有人群都给予P16/Ki-67双染、TCT细胞学、HPV联合检测,分别记录检测阳性情况。同时所有人群都进行了病理组织检查,并以此为诊断金标准,判断诊断价值。结果在83例患者中,病理检查为正常组织21例、CIN 1级47例、CIN 2级12例、CIN 3级3例,其中高级别上皮内瘤变占18.07%。83例患者中,正常组织、CIN 1级、CIN 2级、CIN 3级患者的P16/Ki-67双染、TCT细胞学、HPV联合检测阳性率分别为4.76%、10.64%、66.67%、66.67%。P16/Ki-67双染、TCT细胞学、HPV检查单独检测与联合检测对宫颈病变的诊断敏感性分别为66.67%(10/15)、66.67%(10/15)、100.00%(15/15)、66.67%(10/15),诊断特异性分别为91.18%(62/68)、60.29%(41/68)、17.65%(12/68)、91.18%(62/68)。结论P16/Ki-67双染、TCT细胞学、HPV联合检测技术能更准确地发现潜在的高级别病变患者,提高宫颈病变检出率,有很好的应用价值。

Objective To explore and analysis the application values of P16/Ki-67 double staining,thinprep cytological test(TCT)and human Papilloma(HPV)combined detection technology in screening cervical lesions.Methods From April 2022 to April 2023,83 cases patients who voluntarily underwent cervical lesion screening and cervical tissue biopsy at our hospital's gynecological outpatient and inpatient clinics were collected as the study subjects.All patients received P16/Ki-67 double staining,TCT cytology,and HPV combined testing,and their positive results were recorded.At the same time,all populations underwent pathological tissue examinations and used them as the diagnostic gold standard to determine the diagnostic value.Results In the 83 patients,pathological examination showed 14 cases of normal tissue,54 cases of CIN grade 1,12 cases of CIN grade 2,and 3 cases of CIN grade 3,with high-grade intraepithelial neoplasia accounting for 18.07%.The combined positive rates of normal tissue,CIN 1,CIN 2,and CIN 3 were 4.76%,10.64%,66.67%,and 66.67%,respectively.The diagnostic sensitivity of P16/Ki-67 double staining,TCT cytology,and HPV examination for cervical lesions was 66.67%(10/15),66.67%(10/15),100.00%(15/15),and 66.67%(10/15),respectively.The diagnostic specificity was 91.18%(62/68),60.29%(41/68),17.65%(12/68),and 91.18%(62/68),respectively.Conclusion The P16/Ki-67 double staining,TCT cytology,and HPV combined detection technology can more accurately detect potential high-level lesions in patients,improve the detection rate of cervical lesions,and have good application value.