摘要
旨在研究羔羊运输前、后不同处理对其宰后肌肉能量代谢的影响,分析运输导致羔羊应激的潜在机制,为缓解动物运输应激、提高动物福利提供参考。选取身体健康、体重相近的4月龄湖羊公羔60只,随机均分成3组:对照组、电解多维组和新霉素组,每组20只。所有试验羊进行8 h长途运输,运输当天记为第0天,对照组饲喂基础饲粮,电解多维组于运输前2 d至运输后7 d在每只羔羊基础饲粮中添加电解多维375 mg·d^(-1),新霉素组于第0~7天在运输后对每只羔羊进行新霉素治疗200 mg·d^(-1)。在第0、7和14天,每组分别任选5只羔羊屠宰,取背最长肌检测其糖酵解潜力(glycolysis potential, GP)及相关基因和蛋白表达量。结果表明:1)试验处理极显著影响了肌糖原水平(P<0.01),各组大小关系为新霉素组>电解多维组>对照组。时间极显著影响了肌糖原、游离葡萄糖含量和己糖激酶(hexokinase, HK)及乳酸脱氢酶(lactate dehydrogenase, LDH)活性(P<0.01),羔羊背最长肌中肌糖原与游离葡萄糖含量在第7和14天明显高于第0天;HK活性先升高后降低,第7天最高;运输结束后14 d内肌肉LDH活性上升,对照组和新霉素组在第7天最高,电解多维组第14天最高。2)处理显著影响了LKB1的基因表达(P<0.05),各组大小关系为新霉素组>对照组>电解多维组。时间极显著影响了AMPKα1、AMPKα2、LKB1、CPT-1的基因表达(P<0.01),显著影响了ACC基因及LKB1基因及蛋白表达量(P<0.05);各组CPT-1及ACC在运输后14 d内基因表达量上升。综上,羔羊运输前、后添加电解多维可改善肌肉能量代谢状态,抑制运输诱导的AMPK途径激活。
The purpose of this experiment was to study the effects of different treatments before and after transportation on the musle energy metabolism of lambs after slaughter,and to analyze the potential mechanism of lamb stress caused by transportation,so as to provide a reference for alleviating animal transportation stress and improving animal welfare.Sixty 4-month-old healthy Hu sheep male lambs with similar weight were randomly divided into three groups:control group,multivitamin electrolysis group and neomycin group,with 20 lambs in each group.All the experimental lambs were transported for 8 hours,and the day of transportation was recorded as the 0th day.The control group was fed with basal diet,the multivitamin electrolysis group was added with 375 mg·d^(-1)in the basal diet for each lamb from 2 d before transportation to 7 d after transportation,and the neomycin group was treated with neomycin 200 mg·d^(-1)per lamb after transportation from 0 to 7 d.Five lambs in each group were slaughtered on the 0th,7th and 14th day,and the longissimus dorsi muscle was taken to detect its glycolytic potential(GP)and the expression levels of related genes and proteins.The results showed that:1)The treatment significantly affected the muscle glycogen level(P<0.01),and the order of each group was as follows:neomycin group>multivitamin electrolysis group>control group.Time significantly affected the muscle glycogen,free glucose,hexokinase(HK)and lactate dehydrogenase(LDH)activities of the lamb(P<0.01).The muscle glycogen and free glucose levels of the lamb at 7th and 14th day were gradually increased from 0 to 14 days obviously higher than that of the 0th day after transportation.The HK activity of muscle in each group increased firstly and then decreased within 0-14 d after transportation,and reached the highest on the 7th day.The muscle LDH activity increased within 14 days after transportation,reaching the highest in the control group and neomycin group on the 7th day,and the highest in the multivitamin electrolysis group on the 14th day.2)Treatment significantly affected the gene expression of LKB1(P<0.05),and the order of each group was neomycin group>control group>multivitamin electrolysis group.Time extremely significantly affected the m RNA expression levels of AMPKα1,AMPKα2,LKB 1 and CPT-1(P<0.01),and significantly affected ACC gene(P<0.05).With the prolongation of feeding time after transportation,as well as the gene and protein expression levels of AMPKα1,AMPKα2 and LKB1 in lambs of all groups were decreased(P<0.05).After transportation,the gene expression of CPT-1 and ACC in each group increased within 14 days,and the expression was the lowest on the 0th day.In summary,transportation stress triggers the glycolysis mechanism by activating the glycolysis HK enzyme activity,down-regulates the expression of LKB 1 and AMPKα2 to regulate the AMPK pathway and affect energy metabolism,and up-regulates CPT-1 expression to intensify fatty acid oxidation.A adding electrolytic multivitamin before and after transportation can improve muscle energy metabolism and inhibit the activation of AMPK pathway induced by transportation.
作者
陈聪慧
段春辉
杨欣雨
夏翠
郭云霞
纪守坤
严慧
刘月琴
张英杰
CHEN Conghui;DUAN Chunhui;YANG Xinyu;XIA Cui;GUO Yunxia;JI Shoukun;YAN Hui;LIU Yueqin;ZHANG Yingjie(College of Animal Science and Technology,Hebei Agricultural University,Baoding 071000,China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2023年第12期5101-5111,共11页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家肉羊产业技术体系项目(CARS-38)
运输应激干预对羔羊健康及肠道菌群的影响(KY2022029)。
关键词
羔羊
运输应激
电解多维
新霉素
lambs
transportation stress
multivitamin electrolysis
neomycin