四氧化三钴/氧化铈异质结构模拟酶的抗细胞氧化损伤研究

Study on the anti-oxidative effects of Co_(3)O_(4)/CeO_(2) heterostructure catalase-mimetic enzyme

目的探究具有丰富氧空位的四氧化三钴/氧化铈异质结构(Co_(3)O_(4)/CeO_(2)HSs)模拟酶的活性及其降低氧化应激对细胞的损伤。方法在模拟生理条件下(37℃、pH 7.4),分别将Co_(3)O_(4)/CeO_(2)HSs和CeO_(2)纳米片(CeO_(2)NFs)与过氧化氢(H_(2)O_(2))溶液反应,观察其对H_(2)O_(2)的清除能力;在同等条件下,使用溶解氧仪和TMB检测H_(2)O_(2)的分解产物;研究Co_(3)O_(4)/CeO_(2)HSs对鼠源成纤维L929细胞的毒性,以及在氧化应激条件下对L929细胞的保护作用。结果在模拟生理条件下,Co_(3)O_(4)/CeO_(2)HSs能够将H_(2)O_(2)分解为H_(2)O和O_(2),这一催化分解能力高于单一的CeO_(2)NFs;此外,Co_(3)O_(4)/CeO_(2)HSs具有良好的L929细胞相容性,并能有效降低氧化应激对细胞的氧化损伤。结论通过异质结构将两种协同作用的金属氧化物连接成的Co_(3)O_(4)/CeO_(2)HSs,具有更高的催化活性。研究结果为Co_(3)O_(4)/CeO_(2)HSs及其他具有异质结构的金属氧化物模拟酶在氧化应激方面的生物学应用提供了依据。

OBJECTIVE To investigate the enhancement of mimetic enzyme activity by Co_(3)O_(4)/CeO_(2) heterostructure(Co_(3)O_(4)/CeO_(2) HSs)with rich oxygen vacancies and to reduce the cellular damage by oxidative stress.METHODS Co_(3)O_(4)/CeO_(2) HSs and CeO_(2) nanoflakes(CeO_(2) NFs)were directly reacted with hydrogen peroxide(H_(2)O_(2))under simulated physiological conditions(37℃,pH 7.4),respectively,and their ability to scavenge H_(2)O_(2)was explored.The decomposition products of H_(2)O_(2) were probed using dissolved oxygen meter and TMB under the same conditions.The cytotoxicity of Co_(3)O_(4)/CeO_(2) HSs on L929 cells and its protective effect on L929 cells under oxidative stress conditions were investigated.RESULTS Under simulated physiological conditions,Co_(3)O_(4)/CeO_(2) HSs was able to decompose H_(2)O_(2) into H_(2)O and O_(2),and this catalytic decomposition ability was higher than that of single CeO_(2) NFs,and Co_(3)O_(4)/CeO_(2) HSs had good cytocompatibility and promoted the proliferation of L929 cells,as well as reduced oxidative damage to cells by oxidative stress.CONCLUSION Linking two synergistic metal oxides by heterostructure provides higher catalytic activity.The study provides scientific support for the biological application of Co_(3)O_(4)/CeO_(2) HSs and other metal oxide-based mimetic enzymes with heterostructure in oxidative damage.