系统性红斑狼疮患者外周血肽基脯氨酰顺反异构酶1与白细胞介素-17的分析及其临床意义

Analysis and Clinical Significance of Pin1 and IL-17 in Peripheral Blood of Patients with Systemic Lupus Erythematosus

目的:探讨系统性红斑狼疮(SLE)患者外周血肽基脯氨酰顺反异构酶1(Pin1)活性的表达与细胞因子白细胞介素-17(IL-17)水平相关性及其临床意义。方法:选取98例SLE患者,其中42例为SLE初治患者,另选43例健康体检者为健康对照组。采用酶联免疫吸附试验检测所有研究对象入组时及98例SLE患者治疗后3个月血清中Pin1、IL-17水平,分析98例SLE患者血清Pin1水平变化及与细胞因子IL-17、SLE疾病活动指数(SLEDAI)评分、实验室指标的相关性,分析42例SLE患者初治时、治疗后3个月时其水平变化及意义。结果:①SLE稳定组外周血的Pin1表达水平明显高于健康对照组(H=28.913,P<0.05),SLE活动组外周血的Pin1表达水平明显高于SLE稳定组(H=31.738,P<0.05)。②SLE患者Pin1与IL-17、SLEDAI评分呈正相关(r=0.640,P<0.05;r=0.461,P<0.05),与抗ds-DNA抗体、C反应蛋白、红细胞沉降率无明显相关性。③SLE初治组外周血的Pin1表达水平明显高于健康对照组(H=55.568,P<0.05),SLE初治组治疗后3个月的Pin1表达水平较治疗前下降(H=28.136,P<0.05)。④血清Pin1水平在有肾脏损害的SLE患者表达增高,差异有统计学意义(P<0.05)。结论:SLE患者外周血Pin1过表达,IL-17增高且与其呈正相关,经过治疗能显著降低Pin1活性,降低IL-17细胞因子水平,抑制Pin1蛋白表达,可能会延缓SLE的进展,Pin1抑制剂可能成为SLE治疗的新靶点之一。

Objective:To explore the correlation between the expression of Pin1 activity and cytokine interleukin-17(IL-17)levels in peripheral blood of patients with systemic lupus erythematosus(SLE)and its clinical significance.Methods:Ninety-eight SLE patients were selected,of which 42 cases were newly treated SLE patients,and another 43 healthy examinees were selected as the healthy control group.Enzyme Linked Immunosorbent Assaywas used to detect the levels of Pin1 and IL-17 in the serum of all study subjects at enrollment and 3 months after treatment in 98 SLE patients.The changes in serum Pin1 levels in 98 SLE patients and their correlation with cytokine IL-17,SLE disease activity index(SLEDAI)score,and laboratory indicators were analyzed.The changes and significance of these levels in 42 SLE patients at initial treatment and 3 months after treatment were also analyzed.Results:①The expression level of Pin1 in peripheral blood of the SLE stable group was significantly higher than that of the healthy control group(H=28.913,P<0.05),and the expression level of Pin1 in perpheral blood of the SLE active group was significantly higher than that in the SLE stable group(H=31.738,P<0.05).②Pin1 is positively correlated with IL-17 and SLEDAI scores in SLE patients(r=0.640,P<0.05;r=0.461,P<0.05),but not significantly correlated with anti ds-DNA antibodies,C-reactive protein,and erythrocyte sedimentation rate.③The expression level of Pin1 in peripheral blood of the SLE initial treatment group was significantly higher than that of the healthy control group(H=55.568,P<0.05),and the expression level of Pin1 in the SLE initial treatment group decreased after 3 months'treatment compared to before treatment(H=28.136,P<0.05).④The expression of serum Pin1 level increased in SLE patients with renal damage,and the difference is statistically significant(P<0.05).Conclusion:Pin1 in peripheral blood is overexpressed in SLE patients,and IL-17 is increased and positively correlated with it.Treatment can significantly reduce Pin1 activity,lower IL-17 cytokine levels,and inhibit Pin1 protein expression,which may delay the progression of SLE.Pin1 inhibitors may become one of the new targets for SLE treatment.