中国莫勒菌代谢物的生物活性评价

Bioactivity evaluation of metabolites from Moelleriella sinensis

本文对中国莫勒菌(Moelleriella sinensis)天然产物的生物活性展开初步研究,旨在为合理开发该菌生物资源提供科学依据.对分离纯化到的20个纯化合物进行抗菌活性评价.化合物14对大肠杆菌和青枯劳尔氏菌的最小抑菌浓度(MIC)分别为1.30和6.25μg·mL^(-1),超过阳性对照链霉素.化合物11对大肠杆菌的MIC为6.25μg·mL^(-1),化合物11对5个菌株(大肠杆菌、西瓜枯萎病菌、青枯劳尔氏菌、金黄色葡萄球菌、枯草芽孢杆菌)均有抑制活性,MIC为6.25~25.00μg·mL^(-1),展示了较为广泛的抑菌活性.采用4种方法(超氧阴离子清除能力、DPPH自由基清除能力、ABTS自由基清除能力、T-AOC总抗氧化能力)对分离纯化得到的化合物1~20进行抗氧化活性评价,化合物3、8、11、19表现出较强的抗氧化活性.化合物19对超氧阴离子自由基的清除率最高(在50μg·mL^(-1)时抑制率为7.15%),化合物3对DPPH、ABTS自由基的清除率最高(在50μg·mL^(-1)时清除率分别为66.02%、30.75%),化合物19的总抗氧化能力最强(50μg·mL^(-1)相当于0.63 mmol·L^(-1) FeSO_(4)的抗氧化能力).

We conducted a preliminary study on the biological activity of natural products isolated from Moelleriella sinensis,with the aim to provide scientific basis for the rational development of microbial resources.The antimicrobial activities of 20 purified compounds were evaluated in the present study.Compound 14 exhibited strong antibacterial activity against Escherichia coli with a minimum inhibitory concentration(MIC)of 1.30μg·mL^(-1) and against Ralstonia solanacearum with a MIC of 6.25μg·mL^(-1),respectively,exceeding the values obtained for the positive control,streptomycin.Compound 11 exhibited inhibitory activities against five strains(E.coli,Fusarium oxysporum f.sp.niveum,R.solanacearum,Staphylococcus aureus,and B.subtilis),with MIC values ranging 6.25-25.00μg·mL^(-1),thereby demonstrating a wide range of antimicrobial activities.Four methods,namely superoxide anion scavenging capacity,2,2-diphenyl-1-picrylhydrazyl(DPPH)radical scavenging capacity,2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate)(ABTS)radical scavenging capacity,and T-AOC total antioxidant capacity,were used to evaluate the antioxidant activities of compounds 1-20.Compounds 3,8,11,and 19 showed antioxidant activities.Compound 19 exhibited the highest scavenging rate of superoxide anion radical(inhibition of 7.15%at 50μg·mL^(-1)),whereas compound 3 showed the highest scavenging rate of DPPH and ABTS radical(inhibitory rate of 66.02%and 30.75%at 50μg·mL^(-1),respectively).Moreover,compound 19 had the strongest total antioxidant capacity(50μg·mL^(-1) was equivalent to 0.63 mmol·L^(-1) FeSO_(4)).