植物乳植杆菌CCFM1296及后生元对小鼠透明质酸和衰老的影响

Effects of Lactiplantibacillus plantarum CCFM1296 and its postbiotics on mice hyaluronic acid content and senescence

衰老伴随着透明质酸(hyaluronic acid,HA)的流失,从而出现皮肤衰老、眼花、关节疼痛和记忆衰退等症状,该研究拟探讨口服不同植物乳植杆菌活菌和制备的后生元对体内HA的调控作用和抗衰老作用。通过皮下注射500 mg/(kg·d)D-半乳糖诱导衰老小鼠模型,并每天分别灌胃5×10^(9)CFU/kg植物乳植杆菌CCFM1295、CCFM1296、CCFM1297活菌(CCFM1295-L、CCFM1296-L、CCFM1297-L)和500 mg/kg剂量的后生元(CCFM1295-D、CCFM1296-D、CCFM1297-D),9周后检测小鼠皮肤、关节、脑、眼的HA含量,并对HA合成相关酶水平进行了定量,并检测小鼠中与衰老相关的血清生化指标、皮肤水分和弹性以及抗氧化水平。结果显示,在灌胃植物乳植杆菌小鼠中,仅有CCFM1296-L和CCFM1296-D促进了皮肤、关节、眼的HA含量并促进了组织中HAS2 mRNA的表达水平,CCFM1296-D同时还上调了脑中HAS2 mRNA的表达水平,HAS2是HA生物合成的关键酶之一,其表达水平可能影响HA的水平。同时CCFM1296-L和CCCFM1296-D增加了胆碱酯酶(cholinesterase,CHE)活性并降低了血清C反应蛋白(C-reactive protein,CRP)、谷丙转氨酶(alanine transaminase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)、碱性磷酸酶(alkaline phosphatase,ALP)、肌酸激酶(creatine kinase,CK)水平。CCFM1296-L还能够增加皮肤水分和皮肤弹性,并提高皮肤组织的CAT、GSH-Px和T-AOC活力,增加其防御能力。总之,植物乳植杆菌CCFM1296能够通过上调HAS2 mRNA的表达水平增加组织HA含量,从而帮助机体起到抵抗衰老的作用。

Aging is accompanied by the loss of hyaluronic acid(HA),which causes health problems such as skin aging,dazzling,joint pain,and memory decline.This study intended to explore the regulation of HA and anti-aging effects by oral Lactiplantibacillus plantarum CCFM1296 live bacteria and prepared postbiotics in vivo.The aging mouse model was induced by subcutaneous injection of 500 mg/(kg·d)D-galactose,and 5×109 CFU/kg Lactiplantibacillus plantarum CCFM1295,CCFM1296,CCFM1297 live bacteria(CCFM1295-L,CCFM1296-L,and CCFM1297-L)and 500 mg/kg dose of postbiotics prepared by L.plantarum CCFM1295,CCFM1296,CCFM1297(CCFM1295-D,CCFM1296-D,and CCFM1297-D)were administered daily for about 9 weeks.The content of HA in skin,joints,brain,and eyes of mice was measured and the mRNA expression of enzymes related to HA synthesis were quantified.In addition,aging-related serum biochemical indexes,skin moisture and elasticity,and skin antioxidant levels of mice were also detected.The results showed that both CCFM1296-L and CCFM1296-D promoted the content of HA in skin,joints,and eyes,and stimulated the expression level of HAS2 mRNA in tissues.CCFM1296-D also accelerate HA content in the brain and up-regulated the expression level of HAS2 mRNA.HAS2 is one of the key enzymes for HA biosynthesis,and may affect the level of HA.Further test found that CCFM1296-L and CCCFM1296-D increased CHE activity and decreased serum CRP,ALT,AST,ALP,and CK levels.CCFM1296-L can also increase skin moisture and skin elasticity,and improve the activity of CAT,GSH-Px and T-AOC in skin tissue to increase its defense ability.In general,L.plantarum CCFM1296 and prepared postbiotics can increase tissue HA level by up-regulating the expression level of HAS2 mRNA,thereby assisting the body to counteract aging.