添加植物乳杆菌、布氏乳杆菌对杂交狼尾草青贮发酵品质及有氧稳定性的影响

Effects of Adding Lactiplantibacillus plantarum and Lactobacillus buchneri on Silage Fermentation Quality and Aerobic Stability of Pennisetum glaucum×purpureum

为探究植物乳杆菌、布氏乳杆菌对杂交狼尾草(Pennisetum glaucum×purpureum)青贮发酵品质及有氧稳定性的影响,本试验以杂交狼尾草为试验材料,设置对照组(CK组,无添加)、植物乳杆菌(Lactiplantibacillus plantarum)组(LP组,1×10^(6)cfu·g^(-1)FW)、布氏乳杆菌(Lactobacillus buchneri)组(LB组,1×10^(6)cfu·g^(-1)FW)、植物乳杆菌和布氏乳杆菌组(LPLB组,5×10^(5)cfu·g^(-1)FW LP+5×10^(5)cfu·g^(-1)FW LB),厌氧发酵60天(d)后,测定其营养成分、发酵品质和有氧稳定性。结果表明:与CK组相比,各处理组粗蛋白(CP)、乳酸(LA)和乙酸(AA)含量显著增加(P<0.05),pH值和丁酸(BA)含量显著下降(P<0.05);在有氧暴露阶段,各处理组乳酸(LA)含量显著增加(P<0.05),氨态氮含量显著降低(P<0.05),LB和LPLB组在有氧暴露9天时,酵母菌和好氧菌数量(P<0.05)显著降低,乳酸菌数量显著增加(P<0.05);用隶属函数法进行综合价值评定,LPLB组青贮效果最佳。

The aim of study was to explore the effects of Lactiplantibacillus plantarum and Lactobacillus buchneri on the silage fermentation quality and aerobic stability of Pennisetum glaucum×purpureum.Four groups were established as follows:CK group(no addition),Lactobacillus plantarum group(LP group,1×10^(6)cfu·g^(-1)FW),Lactobacillus buchneri group(LB group,1×10^(6)cfu·g^(-1)FW),and Lactiplantibacillus plantarum and Lactobacillus buchneri group(LPLB group,5×10^(5)cfu·g^(-1)LP+5×10^(5)cfu·g^(-1)LB).After 60 days of fermentation,the fermentation quality and aerobic stability of all the Pennisetum glaucum×purpureum silage were measured.The results showed that:Compared with the CK group,the addition of Lactic acid bacteria significantly improved the crude protein(CP)content in all treatment groups(P<0.05).The pH value and butyric acid(BA)content of Lactic acid bacteria treatment were significantly lower than those of CK group(P<0.05),while the contents of lactic acid(LA)and acetic acid(AA)were significantly higher than those of CK group(P<0.05).During the aerobic exposure,the content of LA was significantly higher than that of CK group(P<0.05).The content of ammonia nitrogen was lower than that of CK group(P<0.05).After 9 days of aerobic exposure,yeast and aerobic bacteria numbers significantly reduced,while lactic acid bacteria numbers significantly increased in LB and LPLB groups(P<0.05).The comprehensive value evaluation by the membership function method found that LPLB group had the best comprehensive performance of silage effect.