LED光照对灰茶尺蠖成虫保护酶活性的影响

Effects of LED light on the activities of protective enzymes in Ectropis grisescens (Lepidoptera: Geometridae) adults

【目的】探讨LED光照波长、强度及处理时间对灰茶尺蠖Ectropis grisescens成虫保护酶活性的影响,为茶园灰茶尺蠖应用灯光绿色防控提供参考。【方法】室内检测了灰茶尺蠖雌雄成虫在LED光照波长590~595和520~525 nm,光照强度分别为40,80和120 lx下照射1,2和3 h时总蛋白含量、以及保护酶[过氧化物酶(peroxidase,POD)、超氧化物歧化酶(superoxide dismutase,SOD)和过氧化氢酶(catalase,CAT)]活性及总抗氧化能力(total antioxidant capacity,T-AOC)的变化。【结果】590~595和520~525 nm两种波长LED光处理后随着光照强度和处理时间的增加,灰茶尺蠖雌雄成虫中总蛋白含量,POD,SOD和CAT活性及T-AOC呈增加趋势。两种波长各光照强度处理不同时间雌成虫中总蛋白含量和POD活性均显著高于雄成虫中的,雄成虫中SOD和CAT活性及T-AOC均高于雌成虫中的,且均以120 lx LED光照强度处理3 h时最高。两个波长的LED光照射不同时间时灰茶尺蠖雌雄成虫体内POD,SOD和CAT活性均高于自然光对照。【结论】590~595和520~525 nm的LED光照均可引起灰茶尺蠖成虫体内保护酶活性变化,为探索打破保护酶活性最佳照射波长、强度和时间及茶园内采用灯光照射茶园害虫开展绿色防控提供基础数据。

【Aim】To study the effects of wavelength,intensity and treatment time of LED light on the activities of protective enzymes in Ectropis grisescens adults,so as to provide references for the green control of E.grisescens with light in tea garden.【Methods】The female and male adults of E.grisescens were exposed to LED light at the wavelengths of 590-595 and 520-525 nm under the light intensities of 40,80 and 120 lx,respectively,in the laboratory,and the changes in the total protein content,activities of protective enzymes[peroxidase(POD),superoxide dismutase(SOD)and catalase(CAT)],and total antioxidant capacity(T-AOC)were tested at 1,2 and 3 h after treatments.【Results】After exposure to LED light at the wavelengths of 590-595 and 520-525 nm,the total protein content,the activities of POD,SOD and CAT,and T-AOC in female and male adults of E.grisescens increased with the increase of light intensity and treatment time.At different time after exposure to LED light at the two wavelengths under various light intensities,the total protein content and POD activities in female adults were significantly higher than those in male adults,and the activities of SOD and CAT,and T-AOC in male adults were higher than those in female adults and those in female and male adults at 3 h under LED light intensity of 120 lx were the highest.The activities of POD,SOD and CAT in female and male adults of E.grisescens subjected to LED light radiation at the two wavelengths for different time was higher than those in the natural light control.【Conclusion】LED light at the wavelengths of 590-595 and 520-525 nm can cause changes in the protective enzyme activities in E.grisescens adults,providing basic data for exploring the best wavelength,intensity and time of breaking protective enzyme activity and green control using light to irradiate pests in tea garden.