DNA/RNA分子中的3′,5′-磷酸二酯键之刍议

Discussion on 3′,5′-phosphodiester bonds in DNA/RNA

3′,5′-磷酸二酯键是生物学、生物化学及有机化学等相关学科中一个重要名词和考点,然而对其释义及内涵的理解在国内外各教材、文献及网络平台上却不同,长期以来是一个争论热点。现利用基团和化学键的基本概念、酯化反应机理及影响因素、磷酸的结构等知识,结合3′,5′-磷酸二酯键的形成机制即核苷酸从头合成途径和核酸合成的相关过程,对3′,5′-磷酸二酯键进行系统剖析和探讨,明确其本质及内涵,体现教学要注重多学科知识的交融和思维的培养。

3′,5′-phosphodiester bond is an important term and topic in biology,biochemistry,organic chemistry and other related subjects.However,the understanding of its interpretation and connotation are different in various textbooks,literatures and online platforms at home and abroad,which has been a hot topic of debate for a long time.Based on the basic concepts of groups and chemical bonds,esterification reaction mechanism and influencing factors,structure of phosphoric acid,combined the formation mechanism of 3′,5′-phosphate diester bonds,videlicet,the de novo synthesis pathway of nucleotides and the related processes of nucleic acid synthesis,this paper systematically analyzes and explores 3′,5′-phosphate diester bonds,clarifies their essence and connotation,and reflects the need for teaching to attach importance to the integration of interdisciplinary knowledge and the cultivation of thinking.