壳寡糖与五种抗生素对常见致病菌的体外协同抑菌效果研究

Study on the in vitro synergistic antibacterial ffects of chito-oligosaccharides with five antibiotics on common pathogenic bacteria

为了测定壳寡糖对常见致病菌的抑制活性及壳寡糖与五种常见抗生素的协同抑菌效果,试验测定了壳寡糖与硫酸链霉素、盐酸四环素、四环素、盐酸强力霉素、硫酸新霉素对大肠杆菌CICC21482、金黄色葡萄球菌ATCC6538P、副溶血弧菌ATCC17802、哈维氏弧菌ATCCCBAA-1117的最小抑菌浓度(minimum inhibitory concentration,MIC)和最小杀菌浓度(minimum bactericidal concentration,MBC),并根据部分抑菌浓度指数(fractional inhibitory concentration index,FIC指数)对壳寡糖与抗生素的联合抑菌效果进行了分析。结果表明:壳寡糖对常见致病菌有较强的抑制活性,其MIC在22.5~45 mg/mL之间和MBC在45~90 mg/mL之间;硫酸新霉素对副溶血弧菌ATCC17802没有抑制作用,未检测到MIC和MBC。因此未进行壳寡糖和硫酸新霉素对副溶血弧菌ATCC17802的联合药敏试验。壳寡糖除与硫酸链霉素、四环素联用对副溶血弧菌ATCC17802表现为无关作用外,与硫酸链霉素、盐酸四环素、四环素、盐酸强力霉素、硫酸新霉素联用表现为部分协同作用和协同作用,协同抑菌效果明显。说明壳寡糖在单独和联合使用时对常见致病菌具有抑制和杀灭作用。

In order to determine the inhibitory activity of chito-oligosaccharides against common pathogens and the synergistic antibacterial effects of chito-oligosaccharides with five common antibiotics,in this experiment,the minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of chito-oligosaccharides and streptomycin sulfate,tetracycline hydrochloride,tetracycline,doxycycline hydrochloride,neomycin sulfate against Escherichia coli CICC21482,Staphylococcus aureus ATCC6538P,Vibrio parahaemolyticus ATCC17802,and Vibrio Harveyi ATCCCBAA-1117 were determined.The synergistic antibacterial effect of chito-oligosaccharides and antibiotics was analyzed according to the fractional inhibitory concentration index(FIC index).The results showed that chito-oligosaccharides had strong inhibitory activity against common pathogenic bacteria,and their MICs were between 22.5-45 mg/mL and MBCs between 45-90 mg/mL.The inhibitory effect of neomycin sulfate on Vibrio parahaemolyticus ATCC17802 was not observed,and no MIC and MBC were detected.Therefore,the combined drug sensitivity test of chito-oligosaccharides and neomycin sulfate on Vibrio parahaemolyticus ATCC17802 was not conducted.Chito-oligosaccharides showed no correlation with streptomycin sulfate and tetracycline on Vibrio parahaemolyticus ATCC17802,but showed partial synergistic and synergistic effects with streptomycin sulfate,tetracycline hydrochloride,tetracycline hydrochloride,doxycycline hydrochloride and neomycin sulfate,and had obvious synergistic antibacterial effect.The results suggested that chito-oligosaccharides had inhibitory and bactericidal effect on common pathogenic bacteria when used alone and in combination.