高效液相色谱法同时测定化妆品中7种禁用植物原料标志性成分

Determination of 7 signature ingredients from prohibited raw material by HPLC

建立高效液相色谱法同时测定化妆品的巴豆苷、苦杏仁苷、花椒毒素、狼毒乙素、马兜铃酸、欧前胡素、土木香内酯7种化合物。采用资生堂CAPCELL PAK MG C18柱(250 mm×4.6 mm,5μm),以乙腈-0.05%磷酸为流动相梯度洗脱,流量为1 mL/min,进样体积为10μL,柱温为30℃,采用二极管阵列检测器,在210 nm波长处测定。7种化合物在所测浓度范围内有良好的线性关系,相关系数均大于0.999,平均回收率为90.0%~105.0%,测定结果的相对标准偏差为0.3%~2.8%(n=6),方法检出限为0.02~0.17μg/g。该方法可以高效、准确的检测化妆品中7种禁用植物原料的标志性成分。

A high-performance liquid chromatography method was developed for rapidly detecting seven compounds including Crotonoside,Amygdalin,xanthotoxin.2,4-dihydroxy-6-methoxy-3 methylacetophenone,aristolochic acid,imperatorin and alantolactone.The analysis was carried out by Capcell PAK MGⅡC18 column(250 mm×4.6 mm,5μm),acetonitrile-water(containing 0.05%phosphoric acid)used as mobile phases for gradient elution,the flow rate was 1.0 mL/min.The injection volume was 10μL,the column temperature was 30℃,and the diode array detector was used,while the wavelength of 210 nm was selected for detection.The method had good linearity of 7 components in corresponding liner range with the correlation coefficients more than 0.999.The recoveries at low and high spiking levels were 90.0%-105.0%with the relative standard deviations ranged from 0.3%to 2.8%.The method detection limits were within the range of 0.02-0.17μg/g.This method was efficient,accurate for the determination of 7 signature ingredients from prohibited raw material.