酒萸肉中2个新的苯丙素类成分

Two new phenylpropanoids from wine-processed Corni Fructus

综合运用硅胶、ODS、Sephadex LH-20柱色谱和反相制备型HPLC等多种分离技术,结合HR-ESI-MS、UV、IR、NMR、ECD等波谱学方法,从酒萸肉水提液中共分离鉴定出6个化合物,分别为pinoresinoside B(1)、cornusgallicacid A(2)、(+)-isolariciresinol-9′-O-β-glucopyranoside(3)、(-)-isolariciresinol 3α-O-β-D-glucopyranoside(4)、(7R,8S)-dihydrodehydrodiconiferyl alcohol 9-O-β-D-glucopyranoside(5)、开环异落叶松脂素-9′-O-β-D-葡萄糖苷(6)。其中化合物1和2为新化合物。生物活性评价结果表明,化合物2和6具有较强的DPPH自由基清除能力,其EC_(50)分别为(4.18±1.96)、(21.45±1.19)μmol·L^(-1);化合物1和2对H_(2)O_(2)诱导的大鼠肾细胞(NRK-52E)氧化损伤均具有保护作用,且呈剂量依赖性,其中化合物2在100μmol·L^(-1)浓度下的细胞存活率达96.09%±1.77%。

Six compounds were isolated from aqueous extract of wine-processed Corni Fructus through silica gel,ODS column chromatography,Sephadex LH-20 gel column chromatography,reverse phase preparative HPLC and other chromatographic separation technologies.Their structures were identified with multiple spectroscopical methods including HR-ESI-MS,UV,IR,NMR and ECD and so on.Their structures were established as pinoresinoside B(1),cornusgallicacid A(2),(+)-isolariciresinol-9′-O-β-glucopyranoside(3),(-)-isolariciresinol 3α-O-β-D-glucopyranoside(4),(7R,8S)-dihydrodehydrodiconiferyl alcohol 9-O-β-D-glucopyranoside(5),and(-)-seco isolariciresinol-9′-O-β-D-glucopyranoside(6).Among them,compounds 1 and 2 were two new compounds.The biological activity evaluation results showed that compounds 2 and 6 had strong DPPH free radical scavenging ability,with EC_(50) values of(4.18±1.96) and(21.45±1.19) μmol·L~(-1),respectively.Compounds 1 and 2 had protective effects on H_2O_2-induced oxidative damage in NRK-52E cells in a dose-dependent manner,and the cell survival rate of compound 2 at 100 μmol·L~(-1) was 96.09%±1.77%.