凉血退紫合剂中8种成分在正常和过敏性紫癜大鼠的组织分布比较研究

Comparison of distribution of eight components from Liangxue Tuizi Mixture between normal and Henoch-Schonlein purpura rats

该研究采用UPLC-TQ-MS技术,以灌胃热性药和注射卵白蛋白与弗氏完全佐剂乳化液的方法复制过敏性紫癜大鼠模型,测定灌胃凉血退紫合剂42 g·kg^(-1)后正常生理状态与过敏性紫癜模型状态下,8种主要成分(阿魏酸、咖啡酸、新绿原酸、隐绿原酸、苯甲酰氧化芍药苷、络石苷、马钱苷、芍药苷)在0.5、1、2、6、12 h大鼠肝、肺、心、脾、肾组织分布差异,探讨其在机体组织器官的分布规律及特性。结果表明,凉血退紫合剂中8种成分在正常和过敏性紫癜模型大鼠各组织中分布行为不同,牡丹皮及白芍中的主要成分芍药苷在各组织中含量均较高;8种成分在肝、肺、肾组织分布较多,脾、心组织次之。

This study used UPLC-TQ-MS technology to replicate a Henoch-Schonlein purpura(HSP) model in rats by administering warm drugs by gavage and injecting ovalbumin with Freund′s complete adjuvant emulsion.The distribution differences and characteristics of eight major components(ferulic acid,caffeic acid,neochlorogenic acid,cryptochlorogenic acid,benzoyl oxypaeoniflorin,tracheloside,loganin,and paeoniflorin) in rat liver,lung,heart,spleen,and kidney tissues were determined after oral administration of the Liangxue Tuizi Mixture at a dose of 42 g·kg~(-1) in both normal physiological and HSP states at 0.5,1,2,6,and 12 hours.The results showed that the distribution patterns of the eight components of Liangxue Tuizi Mixture in the tissues of normal and HSP model rats were different.The main component,paeoniflorin,in Moutan Cortex and Paeoniae Radix Alba had higher content in all tissues.The eight components were predominantly distributed in the liver,lung,and kidney tissues,followed by spleen and heart tissues.