益母草注射液通过其生物碱成分上调Tie-2、VEGFR2和VEGF促进斑马鱼血管新生

Motherwort Injection Promotes Angiogenesis Via Alkaloid-Induced Upregulation of Tie-2,VEGFR2 and VEGF in Zebrafish Modle

目的:研究益母草注射液在斑马鱼血管损伤模型中的促血管生成作用,并阐明潜在的物质基础和分子机制。方法:采用HPLC-PAD和TLC技术检测益母草注射液的主要成分;利用舒尼替尼诱导转基因Tg(flk1:eGFP)斑马鱼血管损伤模型,益母草注射液干预治疗后,测量斑马鱼节间血管(ISVs)长度,QT-PCR检测血管生成相关基因的表达水平。结果:发现益母草注射液的主要成分是水苏碱、胆碱和葫芦巴碱;其中,益母草注射液、葫芦巴碱和胆碱可减轻化学诱导的斑马鱼血管损伤,其作用机制涉及益母草注射液上调Tie-2、VEGF和VEGFR2的表达,其主要成分葫芦巴碱上调Tie-2和VEGF的表达,胆碱上调VEGF和VEGFR2的表达。结论:益母草注射液及其主要生物碱成分可通过上调Tie-2、VEGFR2和VEGF来促进血管生成。

Objective:To study the angiogenic effects of motherwort injection in a zebrafish vascular injury model,and to clarify the potential material basis and molecular mechanism.Methods:Using HPLC-PAD and TLC techniques to detect the main components of motherwort injection.Using sunitinib induced transgenic Tg(flk1:eGFP)zebrafish vascular injury model,after intervention with motherwort injection,the length of intersegmental blood vessels(ISVs)of zebrafish was measured,and the expression level of angiogenesis-related genes was detected by QT-PCR.Results:It was found that the main components of Leonurus heterophyllus injection were stachydrine,choline and trigonelline.Among them,motherwort injection,trigonelline and choline can alleviate chemically induced zebrafish vascular damage,and its mechanism involves that motherwort injection upregulates the expressions of Tie-2,VEGF and VEGFR2,and its main component trigonelline up-regulates the expressions of Tie-2 and VEGF,and choline up-regulates the expressions of VEGF and VEGFR2.Conclusion:Motherwort injection and its main alkaloid components can promote angiogenesis by up-regulating Tie-2,VEGFR2 and VEGF.