有机物存在下Fe^(2+)对厌氧氨氧化脱氮性能的影响

Effect of Fe^(2+)on Nitrogen Removal Performance of Anammox in the Presence of Organic Matter

为分析有机物存在条件下Fe^(2+)对厌氧氨氧化生物膜反应器脱氮性能的影响,在进水COD为90 mg/L、水力负荷为0.1 m^(3)/(m^(2)·h)的条件下投加Fe^(2+),考察不同Fe^(2+)浓度作用下厌氧氨氧化反应器处理效能的变化情况,并采用最大可能数(MPN)法和高通量测序技术对反应器内的微生物进行分析。结果表明,在Fe^(2+)浓度为0.15 mg/L的条件下,反应器对TN的去除率为82.15%;当Fe^(2+)浓度提高至1.5、3.0和4.5 mg/L时,TN去除率分别增加了4.00%、6.89%和7.77%。MPN法计数结果显示,反应器底部的反硝化菌数量随着Fe^(2+)浓度的增加呈上升趋势;高通量测序结果表明,当进水Fe^(2+)浓度由0.15 mg/L增至3.0 mg/L时,厌氧氨氧化反应器上部生物膜中的厚壁菌门(Firmicutes)、浮霉菌门(Planctomycetota)和Denitratisoma属的丰度分别由7.80%、4.87%、4.59%增至10.69%、5.73%、12.25%。一定浓度的Fe^(2+)能够通过促进反应器内具有降解有机物、厌氧氨氧化和反硝化作用微生物的生长来提高厌氧氨氧化生物膜反应器在有机物抑制条件下的脱氮性能。

Ferrous ions(Fe^(2+))were added into the influent to investigate the effects of different ferrous ion concentrations on the treatment performance of Anammox reactor when the influent COD concentration and hydraulic loading rate were 90 mg/L and 0.1 m^(3)/(m^(2)·h),and the microbial community in the reactor was analyzed by MPN method and high‑throughput sequencing,so as to reveal the effect of Fe^(2+)on nitrogen removal efficiency of Anammox biofilm reactor in the presence of organic matter.When Fe^(2+)concentration was 0.15 mg/L,the reactor demonstrated removal rate of 82.15%for TN.With the increase of Fe^(2+)concentration to 1.5 mg/L,3.0 mg/L and 4.5 mg/L,the removal rates of TN increased by 4.00%,6.89%and 7.77%,respectively.The MPN counting results showed that the number of denitrifying bacteria at the bottom of the reactor increased with the increase of Fe^(2+)concentration.The results of high‑throughput sequencing showed that the abundance of Firmicutes,Planctomycetota and Denitratisoma in the biofilm of the upper part of the Anammox reactor increased from 7.80%,4.87%and 4.59%to 10.69%,5.73%and 12.25%respectively when the influent Fe^(2+)concentration increased from 0.15 mg/L to 3.0 mg/L.Specific concentration of Fe^(2+)improved the nitrogen removal performance of the Anammox biofilm reactor under organic matter inhibition by promoting the growth of microorganisms capable of organic matter degradation,Anammox and denitrification in the reactor.