抗阻运动调控鸢尾素促进导管相关性血栓溶解再通的研究

Effect and Potential Mechanism of Resistance Exercise on the Dissolution of Catheter-associated Thrombus

目的:探讨抗阻运动对导管相关性血栓(CRT)溶解再通的影响及可能机制。方法:将36只SD雄性大鼠随机分为对照组、CRT组、CRT+抗阻运动组,每组12只。除对照组大鼠外,其余大鼠均构建CRT模型。CRT组不做任何干预,CRT+抗阻运动组大鼠于建模10 d后开始抗阻运动干预,干预28 d后取材。苏木素-伊红染色观察血栓情况,计算血栓溶解率,酶联免疫吸附剂实验(ELISA)检测血清鸢尾素(Irisin)、白细胞介素(IL)-6、IL-10含量,实时荧光定量PCR检测腓肠肌组织过氧化物酶体增殖物激活受体γ辅激活因子1α(PGC-1α)、Ⅲ型纤连蛋白域包含蛋白5(FNDC5)mRNA表达水平。结果:对照组大鼠无血栓形成。与对照组比较,CRT组大鼠血清Irisin、IL-10含量减少,IL-6含量增高(P均<0.01)。与CRT组比较,CRT+抗阻运动组血栓溶解率显著升高(P<0.01),腓肠肌组织PGC-1α、FNDC5 mRNA表达量增加(P均<0.01),血清Irisin与IL-10含量显著增加,血清IL-6含量减少。结论:抗阻运动28 d促进CRT溶解,可能与上调Irisin表达,抑制炎症有关。

Objectives:To investigate the effect of resistance exercise(RE)on the dissolution of catheter-associated thrombus(CRT)and its possible mechanism.Methods:A total of 36 male SD rats were randomly divided into control group,CRT group,CRT+RE group(n=12 in each group).Rats in the CRT group did not receive any intervention,while rats in the CRT+RE group received RE intervention 10 days after modeling for 28 days.The thrombolysis rate was evaluated by Hematoxylin-Eosin staining.The contents of irisin,interleukin(IL)-6 and IL-10 in serum were detected by ELISA.The relative mRNA expression levels of peroxisome proliferator-activated receptorγcoactivatorα(PGC-1α)and fibronectin type III domain-containing 5(FNDC5)in gastrocnemius were detected by real-time quantitative PCR(qPCR).Results:There was no thrombosis in control group.Compared with control group,the contents of irisin and IL-10 in CRT group were significantly decreased,while the content of IL-6 in CRT rats was significantly increased(all P<0.01).Thrombolysis rate was significantly higher,and the mRNA expressions of PGC-1αand FNDC5 in gastrocnemius were significantly upregulated in CRT+RE group than in CRT group(all P<0.01).Serum irisin,IL-10 contents were significantly increased,IL-6 content was significantly lower in CRT+RE group than in CRT group(allP<0.01).Conclusions:Resistance exercise for 28 days could promote the dissolution of CRT,which may be related to the up-regulation of irisin expression and the inhibition of inflammation.