负载鳙鱼肽的壳聚糖/三聚磷酸钠和壳聚糖/亚麻籽胶复合纳米颗粒的稳定性和生物相容性评价

Evaluation of Stability and Biocompatibility of Chitosan/Sodium Tripolyphosphate and Chitosan/Flaxseed Gum Composite Nanoparticles Loaded with Bighead Carp Peptides

壳聚糖纳米颗粒具有制备简单、成本低和生物相容性高等优点,正逐渐成为递送生物活性成分的优良载体。前期研究表明负载鳙鱼肽(bighead carp peptides,BCP)的壳聚糖/三聚磷酸钠(chitosan/sodium tripolyphosphate,CS/TPP)和壳聚糖/亚麻籽胶(chitosan/flaxseed gum,CS/FG)纳米颗粒具有粒径小、包埋率高和缓释效果显著等优点。本研究主要探究离子强度、pH值、模拟消化和贮藏时间对制备的CS/TPP-BCP和CS/FG-BCP纳米颗粒的影响,并以Caco-2细胞为模型,评估壳聚糖纳米颗粒处理的胞外乳酸脱氢酶的含量、体内抗氧化能力和细胞摄取度。结果表明两种壳聚糖纳米颗粒在酸性条件下比较稳定,对带相反电荷的溶液较为敏感。经过纳米颗粒负载后的多肽的稳定性高于游离肽且两种纳米颗粒都具有较高生物相容性以及细胞摄取度。

Chitosan nanoparticle is becoming an excellent carrier for the delivery of bioactive components due to the advantages of simple preparation,low cost and high biocompatibility.Previous studies have shown that chitosan/sodium tripolyphosphate(CS/TPP)and chitosan/flaxseed gum(CS/FG)nanoparticles loaded with bighead carp peptides(BCP)have the advantages of small particle size,high encapsulation rate and significant slow-release effect.This study explored the effects of ionic strength,pH,simulated digestion and storage time on the preparation of chitosan/sodium tripolyphosphate(CS/TPP-BCP)and chitosan/flaxseed gum(CS/FG-BCP)nanoparticles,and evaluated the extracellular lactate dehydrogenase content and antioxidant capacity in vivo of Caco-2 cells treated with the chitosan nanoparticles and their cellular uptake.The results showed that the two kinds of chitosan nanoparticles were stable under acidic conditions and sensitive to a solution with opposite charges.The stability of the nanoparticles loaded with bighead peptides was higher than that of free peptides and both nanoparticles showed higher biocompatibility and cell uptake.