多花黄精提取物体外干预3T3-L1前脂肪细胞并缓解其脂质积累

Polygonatum cyrtonema Hua Extracts Inhibits 3T3-L1 Preadipocytes in Vitro and Alleviates Lipid Accumulation

研究多花黄精提取物对3T3-L1前脂肪细胞以及分化后成熟脂肪细胞的作用。用φ=70%乙醇和复合酶(纤维素酶:木瓜蛋白酶=3:7)为提取剂,分别提取出多花黄精生品乙醇提取物(Raw Products Extract,RPE)、多花黄精九蒸九制品乙醇提取物(Processed Products Extract,PPE)和多花黄精生品多糖(Polysaccharide from Polygonatum cyrtonema Hua,CPP),得到的九蒸九制品提取物中多糖质量百分比减少(RPE 29.83%,PPE 1.92%),但表现出更高的皂苷、总黄酮和总酚含量(13.63%、5.37 mg/g、14.52 mg/g)。各多花黄精提取物处理3T3-L1细胞后,均观察到3T3-L1前脂肪细胞被阻滞在G0/G1和S期,细胞被诱导凋亡从而抑制细胞的增殖,成熟脂肪细胞内脂滴沉积累量和甘油三酯(Triglyceride,TG)所占比例显著降低,且高剂量组的PPE(380μg/mL)对降低TG占比的效果最显著,与市售多花黄精九蒸九制品多糖(Polysaccharides from Purchased Products,PP)无显著差异。此外,脂肪生成和脂肪酸氧化有关基因也可受到调控,包括抑制PPARγ、C/EBPα、FABP4和LPL的mRNA表达,促进CPT-1的mRNA表达。结果提示,多花黄精提取物可防止脂肪细胞在体外积聚脂肪,表明多花黄精可能具有抗肥胖作用。

The effects of Polygonatum cyrtonema Hua extract on 3T3-L1 preadipocytes and differentiated adipocytes were examined.Raw product extract(RPE),processed product extract(PPE),and polysaccharides(CPP)were obtained usingφ=70% ethanol and complex enzymes(cellulase:papain=3:7).Higher saponin,total flavonoid,and total phenol contents(13.63%,5.37 mg/g,14.52 mg/g)were obtained in the extract of processed products.Following treatment of 3T3-L1 cells with the extract,it was observed that the preadipocytes were blocked in the G0/G1 phases,thereby inducing apoptosis and inhibiting cell proliferation;in addition,mature adipocytes exhibited significantly less lipid droplet formation and triglyceride(TG)content.PPE at 380μg/mL had the most remarkable effect on reducing TG content,showing no significant difference compared to commercially available polysaccharide products(PP).Furthermore,the expression of genes associated with lipogenesis and fatty acid oxidation could be regulated,including inhibiting the mRNA expression of PPARγ,C/EBPα,FABP4,and LPL and promoting the mRNA expression of CPT-1.Thus,the findings of this study show that P.cyrtonema extract has the potential to prevent fat accumulation in vitro,suggesting its possible anti-obesity effects.