软儿梨的抗炎抗氧化作用

Anti-inflammatory and Antioxidant Effects of Ruanerli

软儿梨是甘肃特色水果,具有润肺止咳的功效,但对其机制研究甚少。炎症是呼吸道疾病的主要诱因,该研究旨在通过体内体外试验评价软儿梨的抗炎抗氧化作用,为其用于呼吸道疾病提供理论依据。采用小鼠耳肿胀试验,以脏器指数、白细胞数量以及肿胀抑制率为指标,探讨软儿梨抗炎作用;采用大鼠足肿胀试验,以抗氧化酶活性、丙二醛及前列腺素E2含量为指标,探讨软儿梨的抗氧化作用。通过体外自由基清除试验,采用四参数拟合法,探讨软儿梨对羟自由基(OH·)、超氧阴离子自由基(O_(2)^(-)·)和二苯基苦基苯肼自由基(Diphenyl Picrohydrazine,DPPH·)的清除力。结果表明,软儿梨能显著控制因二甲苯而引起的小鼠耳肿胀(高剂量组抑制率达36.16%),降低脏器指数,减少外周血中白细胞总量以及中性粒细胞、单核细胞和淋巴细胞数量;显著降低蛋清致炎大鼠肿胀足掌中前列腺素E2含量和血清丙二醛含量,增强蛋清致炎大鼠血清中抗氧化酶活性。在检测浓度范围内,软儿梨对DPPH、OH^(-)和O_(2)^(-)自由基均表现出较强的清除力,IC_(50)分别为:0.26、0.23和0.27 g/mL。因此,软儿梨具有较好的抗炎抗氧化作用,其机制可能与减少致炎因子产生、增加自由基清除力、增强抗氧化物酶活性有关。

Ruanerli is a characteristic fruit of Gansu and has the function of moistening the lungs and relieving cough,though the underlying mechanism is rarely studied.Inflammation is the main cause of respiratory diseases.The anti-inflammatory and antioxidant effects of Ruanerli through in vitro and in vivo experiments were evaluated,thereby providing a theoretical basis for its application in respiratory diseases.The anti-inflammatory effects of Ruanerli were evaluated by the mouse ear swelling test based on the organ index,number of white blood cells and inhibition rate of ear swelling.The rat foot swelling test was used to examine the antioxidant effect of Ruanerli using the activities of antioxidant enzyme,serum MDA content and PGE2 level as the indices.Through in vitro free radical scavenging tests,using the four-parameter fitting method,the scavenging abilities of Ruanerli against hydroxyl radical(OH·),superoxide anion radical(O_(2)^(-)·)and DPPH radical were evaluated.The results showed that Ruanerli could significantly suppress the ear swelling caused by xylene in mice(the inhibition rate of the high dose group reached 36.16%),reduce the organ index and reduce the total amount of white blood cells,neutrophils,monocytes and lymphocytes in peripheral blood.Ruanerli could significantly reduce the contents of serum PGE2 and serum MDA while enhancing the activities of serum SOD,CAT and GSH-Px in rats with egg white-induced inflammation.Within the analysed concentration range,Ruanerli showed strong scavenging power against DPPH,OH^(-)and O_(2)^(-)free radicals,with the IC_(50) values being 0.26 g/mL,0.23 g/mL and 0.27 g/mL,respectively.Therefore,Ruanerli pear has good anti-inflammatory and antioxidant effects,and the underlying mechanisms may be related to the reduced production of inflammatory factors,increased free radical scavenging abilities,and enhanced activities of antioxidant enzymes.