摘要
谷氨酰胺转氨酶(transglutaminase,TG)是一类应用广泛、具有优秀蛋白交联能力的酶。TG普遍存在于动植物和微生物中,微生物来源的TG(microbial TG,mTG)拥有良好的酶学性质,因此广泛应用于工业化生产和应用中。本文阐述了mTG的理化性质和自身的活化机理,以及利用野生菌株和不同的重组基因工程菌株发酵生产mTG的研究成果及进展,同时综述了mTG在多种工业生产中的应用及潜力,以期为mTG在工业生产和应用方面的研究提供参考和新的思路。
Transglutaminase(TG)is a widely used enzyme with excellent protein cross-linking capacity.TG is commonly found in plants,animals and microorganisms,and microbial TG(mTG)is widely used in industrial production and application because of its good enzymatic properties.This paper describes the physicochemical properties and activation mechanism of mTG,and summarizes recent progress in mTG production by wild and different genetically engineered strains.Meanwhile,the application and potential of mTG in various industrialfields are reviewed.This review is expected to provide a reference and new ideas for research on the potential of mTG for industrial production and application.
作者
闫文慧
孙鑫洁
袭祥恒
王晨莹
任喜东
YAN Wenhui;SUN Xinjie;XI Xiangheng;WANG Chenying;REN Xidong(State Key Laboratory of Biobased Material and Green Papermaking,Qilu University of Technology,Shandong Academy of Sciences,Jinan 250353,China;Shandong Provincial Key Laboratory of Microbial Engineering,Qilu University of Technology,Shandong Academy of Sciences,Jinan 250353,China;School of Food Science and Engineering,Qilu University of Technology,Shandong Academy of Sciences,Jinan 250353,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2023年第23期223-230,共8页
Food Science
基金
国家自然科学基金青年科学基金项目(32201970)
山东省自然科学基金青年项目(ZR2020QC221)
山东省重大科技创新工程项目(2020CXGC010604)
齐鲁工业大学(山东省科学院)-威海市产学研协同创新基金项目(2021CXY-03)
齐鲁工业大学(山东省科学院)科教产融合试点工程基础研究类项目(2023PY014)。
关键词
谷氨酰胺转氨酶
活化机理
发酵
异源表达
应用
transglutaminase
activation mechanism
fermentation
heterologous expression
application
