MZF1通过调控PLA2G6参与肾透明细胞癌的进程

MZF1 Involved in the Progression of Renal Clear Cell Carcinoma by Regulating Pla2G6

目的探讨髓样锌指蛋白1(recombinant human myeloid zinc finger 1,MZF1)在肾透明细胞癌(kidney renal clear cell carcinoma,KIRC)中的潜在临床意义,以及靶向治疗的潜在基因。方法佳木斯大学基础医学院和齐齐哈尔市第一医院自2022年10月—2023年5月从癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库下载TCGA KIRC RNA-Seq基因表达数据和UCSC Xena数据库的临床材料。用荧光定量PCR和Western blot检测MZF1和钙非依赖性磷脂酶A2G6(calcium-independent phospholipase A2G6,PLA2G6)的mRNA和蛋白表达水平。使用细胞计数试剂盒-8(CCK-8)检测细胞生存能力。结果肾脏细胞系HK-2中MZF1的mRNA、蛋白水平明显低于OSRC-2、786-O中mRNA水平,差异有统计学意义(P<0.05)。MZF1si 1组和MZF1si 2组的MZF1 mRNA、蛋白水平明显低于blank组,差异有统计学意义(P<0.05)。OSRC-2与786-O细胞中si-MZF1组OD值均小于si-NC组,差异有统计学意义(P<0.05)。肾脏细胞系HK-2中PLA2G6的mRNA、蛋白水平明显低于OSRC-2、786-O中水平,差异有统计学意义(P<0.05)。与OSRC-2组相比,ov-MZF1 OSRC-2组PLA2G6的mRNA水平明显升高,差异有统计学意义(P<0.05),si-MZF1 OSRC-2组PLA2G6的mRNA水平明显降低,差异有统计学意义(P<0.05)。与OSRC-2组相比,ov-MZF1 OSRC-2组PLA2G6的蛋白水平明显升高,差异有统计学意义(P<0.05),si-MZF1 OSRC-2组PLA2G6的蛋白水平明显降低,差异有统计学意义(P<0.05)。与Empty vector+sh-NC组相比,OE-MZF1+sh-NC组的OD值明显升高,差异有统计学意义(P<0.05),OE-MZF1+sh-PLA2G6组的OD值明显降低,差异有统计学意义(P<0.05)。结论MZF1通过调控PLA2G6参与了KIRC的进程。

Objective To investigate the potential clinical significance of recombinant human myeloid zinc finger 1(MZF1)in kidney renal clear cell carcinoma(KIRC)and the potential gene for targeted therapy.Methods The School of Basic Medicine of Jimusi University and Qiqihar First Hospital downloaded the KIRC RNA-Seq gene expression data of the cancer genome atlas(TCGA)database and clinical materials of the UCSC Xena database from October 2022 to May 2023.The mRNA and protein expression levels of MZF1 and calcium-independent phospholipase A2G6,(PLA2G6)were detected by fluorescence quantitative PCR and Western blot.Cell viability was measured using cell counting Kit 8(CCK-8).Results The mRNA and protein levels of MZF1 in kidney cell line HK-2 were significantly lower than those in OSRC-2 and 786-O,and the difference was statistically significant(P<0.05).The mRNA and protein levels of MZF1 in MZF1si 1 group and MZF1si 2 group were significantly lower than those in blank group,and the difference was statistically significant(P<0.05).The OD values in OSRC-2 and 786-O cells in si-MZF1 group were lower than those in si-NC group,and the difference was statistically significant(P<0.05).The mRNA and protein levels of PLA2G6 in renal cell line HK-2 were significantly lower than those in OSRC-2 and 786-O,and the differences were statistically significant(P<0.05).Compared with OSRC-2 group,PLA2G6 mRNA level in ov-MZF1 OSRC-2 group was significantly increased,and the difference was statistically significant(P<0.05),while PLA2G6 mRNA level in si-MZF1 OSRC-2 group was significantly decreased,and the difference was statistically significant(P<0.05).Compared with OSRC-2 group,the PLA2G6 protein level in ov-MZF1 OSRC-2 group was significantly increased,and the difference was statistically significant(P<0.05),and the PLA2G6 protein level in si-MZF1 OSRC-2 group was significantly decreased(P<0.05).Compared with Empty vector+sh-NC group,OD value of OE-MZF1+sh-NC group was significantly increased,and the difference was statistically significant(P<0.05),and OD value of OEMZF1+sh-PLA2G6 group was significantly decreased,and the difference was statistically significant(P<0.05).Conclusion MZF1 involved in the progression of KIRC by regulating PLA2G6.