^(60)Co-γ辐射对2种锦带幼苗生长及生理指标的影响

Effects of ^(60)Co-γ radiation on growth and physiological index of two kinds of Weigela florida seedlings

为了探究辐射诱变对“白花锦带花”和“锦带花”形态及生理特性的影响,采用不同剂量(0~300 Gy)的^(60)Co-γ射线辐射“白花锦带花”与“锦带花”休眠期种子,观测辐射对锦带花生长的影响,测定其生长量及幼苗叶片的叶绿素含量、叶绿素荧光指标、光合作用参数、SOD、POD、MDA和可溶性蛋白含量。研究结果显示,由于辐射剂量的增大,苗高、叶长、叶宽、节间距、叶绿素荧光、光合作用参数、SOD活性、POD活性出现了明显的先升高后降低的趋势,在50 Gy时到达了峰值。同时,随着辐射剂量的增加,叶绿素含量和可溶性蛋白含量呈现出下降的趋势,MDA含量明显升高。综上所述,在(2 Gy/min)剂量率的辐射条件下“白花锦带花”种子较适宜的诱变剂量为50 Gy,而“锦带花”以50~100 Gy最合适。

s:In order to explore the effects of radiation mutagenesis on the morphological and physiological characteristics of Weigela florida“Alba”and Weigela florida,the ^(60)Co-γ radiation at different doses(0~300 Gy)was used to irradiate the dormant seeds of W.florida“Alba”and W.florida to observe the effect of radiation on the growth of alum root,the growth amount and chlorophyll content,chlorophyll fluorescence,photosynthetic index,SOD,POD,MDA and soluble protein content of seedling leaves were measured.The results showed that due to the increase of radiation dose,seedling height,leaf length,leaf width,pitch spacing,chlorophyll fluorescence,photosynthesis parameters,SOD activity and POD activity showed a clear trend of first increasing and then decreasing,reaching a peak at 50 Gy.At the same time,with the increase of radiation dose,the chlorophyll content and soluble protein content showed a downward trend.The MDA content was significantly increased.In summary,under the radiation condition of(2 Gy/min)dose rate,the mutagenic dose of W.florida“Alba”seeds was 50 Gy,the W.florida in 50-100 Gy was the most suitable.