冬季农田不同深度土层及小麦根际土壤细菌群落结构和共现网络分析

Bacterial community structure and co-occurrence network in different depths of farmland soil and wheat rhizosphere soil in winter

为探究冬季农田不同深度土层及小麦根际土壤细菌的多样性和群落结构,采集不同深度的农田土壤(0≤FG-A≤5 cm、5<FG-B≤10 cm、10<FG-C≤15 cm、15<FG-D≤20 cm)及小麦根际土壤,对细菌16S rRNA的V4区扩增并进行高通量测序,分析土壤样品中细菌的多样性和种群结构。结果表明:在门水平上,变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)、放线菌门(Actinobacteria)和厚壁菌门(Firmicutes)为优势菌门;在属水平上,节杆菌属(Arthrobacter)和Gp6分别在小麦根际和土壤柱状样中占据优势。此外,酸杆菌门丰度与pH值呈正相关,与NH+4-N和TOC含量呈负相关。样本聚类显示GJ、FG-A和FG-B相互聚类。共现网络分析表明,变形菌门、酸杆菌门、拟杆菌门(Bacteroides)在网络中共现性较高,不同模块之间的细菌组成以及相互作用均具有独特性。

To investigate the diversity and community structure of bacteria in different depths of winter farmland soil and wheat rhizosphere soil,different depths of farmland soil layers(0≤FG-A≤5 cm,5<FG-B≤10 cm,10<FG-C≤15 cm,15<FG-D≤20 cm)and wheat rhizosphere soil were collected.The V4 region of bacterial 16S rRNA was expanded and high-throughput sequencing was performed to analyze the diversity and communi-ty structure of bacteria in soil samples.The results showed that at the phylum level,Proteobacteria,Acidobacte-ria,Actinobacteria and Firmicutes are the dominant phylums,and at the genus level,Arthrobacter and GP6 are dominant in wheat rhizosphere and soil column,respectively.In addition,the abundance of Acinetobacter was positively correlated with pH value,and negatively correlated with NH,*-N and TOC content.Sample cluste-ring showed that GJ,FG-A and FG-B are clustered with each other.Co-occurrence network analysis showed that Proteobacteria,Acinetobacter and Bacteroides have high co-occurrence in the network,and the bacterial composition and interaction between different modules are unique.