NLRC3参与的肿瘤免疫机制在子宫内膜异位症发病中的作用

The Role of NLRC3 in the Tumor Immune Mechanism Involved in the Pathogenesis of Endometriosis

目的:探索Ⅲ期、Ⅳ期子宫内膜异位症(endometriosis,EMT)患者卵巢异位内膜组织中核苷酸结合寡聚结构域样受体家族胱天蛋白酶募集结构域蛋白3(NLRC3)的表达及其与临床病理特征的关系。方法:免疫组化、Western Blot、qRT-PCR方法检测17例Ⅲ期、17例Ⅳ期卵巢异位内膜组织和17例正常子宫内膜组织中NLRC3的表达,分析NLRC3与EMT患者临床病理特征之间的关系。结果:与正常子宫内膜组织相比,Ⅲ期、Ⅳ期异位子宫内膜组织中NLRC3表达水平均降低(P<0.05),而NLRC3的表达在Ⅲ期、Ⅳ期子宫内膜异位症患者中差异无统计学意义(P>0.05)。CA125水平高者NLRC3蛋白表达阳性率更低(P<0.05)。不同年龄、不同病灶大小、不同痛经程度EMT患者的NLRC3表达差异无统计学意义(P>0.05)。结论:EMT的发病与腹腔微环境免疫失衡有关,NLRC3发挥的免疫监视功能下降或缺失可能参与EMT的发生发展。联合检测CA125和NLRC3的表达可能为预测或诊断EMT发挥良好的作用。

Objective:To explore the expression of Nucleotide-binding Oligomerization Domain(NOD)-like receptor family CARD domain-containing protein 3(NLRC3)in ovarian ectopic endometrial tissues of stage III and IV endometriosis(EMT)patients and its relationship with clinical pathological features.Methods:Immunohistochemistry,Western Blot,and qRT-PCR were used to detect the expression of NLRC3 in 17 cases of stage III,17 cases of stage IV ovarian ectopic endometrial tissues,and 17 cases of normal endometrial tissues.The relationship between NLRC3 and clinical pathological features of EMT patients was analyzed.Results:Compared with normal endometrial tissues,the expression level of NLRC3 was decreased in stage III and stage IV ectopic endometrial tissues(P<0.05),while there was no statistically significant difference in NLRC3 expression between stage III and stage IV endometriosis patients(P>0.05).The positive rate of NL-RC3 protein expression was lower in patients with higher CA125 levels(P<0.05).There was no statistically significant difference in NLRC3 expression among EMT patients of different ages,lesion sizes,and degrees of dysmenorrhea(P>0.05).Conclusion:The pathogenesis of EMT is associated with immune imbalance in the peritoneal microenvironment,and the decreased or lost immune surveillance function of NLRC3 may be in-volved in the occurrence and development of EMT.The combined detection of CA125 and NLRC3 expression may play a significant role in predicting or diagnosing EMT.