基于GEO数据库的心脏衰老相关生物信息学分析

Bioinformatics Analysis of Cardiac Aging based on GEO Database

目的采用生物信息学方法探究心脏衰老的关键基因。方法从基因表达综合数据库(Gene Expression Omnibus,GEO)中选择基因表达谱GSE8146。通过R软件进行相关分析,筛选出年轻小鼠与老年小鼠心脏中差异表达基因,利用DAVID 6.8在线分析工具对差异表达基因进行基因本体(Gene Ontology,GO)功能富集分析和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)信号通路富集分析。基于STRING在线数据库进行蛋白互作(protein-protein interaction,PPI)网络分析获得关键基因。结果共筛选出55个差异表达基因,其中上调基因22个,下调基因33个。差异表达基因显著富集于脂质代谢、脂肪酸代谢等过程,参与了过氧化物酶体增殖物激活受体(peroxisome proliferators-activated receptor,PPAR)、腺苷酸活化蛋白激酶(adenosine 5′-monophosphate-activated protein kinase,AMPK)信号通路等通路功能。筛选出Fasn、Pck1、Adipoq、Cpt1a、Pdk4、Pnpla2、Slc27a1、Hmgcs2、Cidec、Ucp3等关键基因。结论心脏衰老可能与细胞能量代谢障碍相关,Fasn、Pck1、Adipoq、Cpt1a、Pdk4、Pnpla2、Slc27a1、Hmgcs2、Ucp3等关键基因及PPAR、AMPK信号通路等可能在心脏衰老的发生、发展中发挥重要作用。

Objective To explore the key genes of cardiac aging by bioinformatic analysis,and conduce to prevent and treat cardio-vascular diseases in the elderly.Methods The gene expression of GSE8146 was downloaded from the Gene Expression Omnibus(GEO)database.“R”software was used to screen out differentially expressed genes in the hearts of young and old mice,and DAVID online anal-ysis tool was used for Gene Ontology(GO)function enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)signa-ling pathway enrichment analysis of differentially expressed genes.Hub genes were obtained by protein-protein interaction(PPI)analy-sis based on the STRING online database.Results A total of 55 differentially expressed genes were screened,including 22 up-regula-ted genes and 33down-regulated genes.The differentially expressed genes were significantly enriched in lipid metabolism,fatty acid me-tabolism and other processes,mainly involvded in peroxisome proliferators-activated receptor(PPAR)signaling pathway and Adenosine 5′-monophosphate(AMP)-activated protein kinase(AMPK)signaling pathway.The hub genes such as Fasn,Pck1,Adipoq,Cpt1a,Pdk4,Pnpla2,Slc27a1,Hmgcs2,Cidec,Ucp3 were screened out.Conclusion Cardiac aging may be related to the disorder of cellular energy metabolism,and hub genes such as Fasn,Pck1,Adipoq,Cpt1a,Pdk4,Pnpla2,Slc27a1,Hmgcs2,Ucp3,PPAR signaling path-way and AMPK signaling pathway may play an important role in the development of cardiac aging.