UBE2T在子宫内膜癌中的表达及临床意义

Expression and Clinical Significance of UBE2T in Endometrial Carcinoma

目的研究泛素结合酶E2T(ubiquitin binding enzyme E2T,UBE2T)在子宫内膜癌组织中表达及临床意义。方法通过TCGA数据库分析UBE2T在正常子宫内膜组织与子宫内膜癌组织中的表达情况;采用实时荧光定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)技术检测58例子宫内膜癌组织和30例正常子宫内膜组织中UBE2T mRNA的表达情况。选取105例子宫内膜癌组织、35例不典型增生子宫内膜组织以及35例正常子宫内膜组织中,采用免疫组织化学法检测各组UBE2T蛋白的表达情况,并分析UBE2T表达与患者临床病理特征的关系,采用Kaplan-Meier法分析UBE2T表达对子宫内膜癌患者预后的影响。结果TCGA数据库显示,UBE2T mRNA在不同子宫内膜组织中的表达比较,差异有统计学意义;在配对的子宫内膜癌组织及其癌旁组织中比较,差异有统计学意义。子宫内膜癌组织中UBE2T mRNA表达水平明显高于正常子宫内膜组织。UBE2T蛋白在子宫内膜癌组织中的阳性表达率高于正常子宫内膜组织及不典型增生子宫内膜组织;UBE2T在子宫内膜癌组织中的阳性表达在国际妇产科联盟(International Federation of Gynecology and Obstetrics,FIGO)分期、组织分化程度方面比较,差异有统计学意义(P<0.05)。Kaplan-Meier生存分析结果显示,UBE2T阳性表达患者的平均生存时间短于阴性表达患者。COX回归分析结果显示,FIGO分期、淋巴结转移及UBE2T的表达是子宫内膜癌患者预后的影响因素,其中FIGO分期、UBE2T的表达是影响子宫内膜癌预后的独立危险因素。结论子宫内膜癌组织中UBE2T表达水平增高,与子宫内膜癌的发生、发展和不良预后密切有关,有可能成为子宫内膜癌的辅助治疗。

Objective To investigate the expression and clinical significance of UBE2T in endometrial carcinoma.Methods The expression of ubiquitin binding enzyme E2T(UBE2T)in normal endometrial tissues and endometrial carcinoma tissues was analyzed by TCGA database.The expression of UBE2T mRNA in 30 cases of normal endometrium and 58 cases of endometrial carcinoma was detected by real-time quantitative polymerase chain reaction(RT-qPCR).105 cases of endometrial carcinoma tissues,35 cases of endometrial atypical hyperplasia tissues and 35 cases of normal endometrial tissues were selected.Immunohistochemistry was used to detect the expression of UBE2T protein in each group,and the relationship between UBE2T expression and clinicopathological features was analyzed.Kaplan-Meier analysis was used to analyze the effect of UBE2T expression on the prognosis of patients with endometrial carcinoma.Results TCGA database showed that the expression of UBE2T mRNA in different endometrial tissues was significantly different.There were statistically significant differences in paired endometrial carcinoma tissues and its adjacent tissues.The expression level of UBE2T mRNA in endometrial carcinoma tissues was significantly higher than that in normal endometrial tissues.The positive expression rate of UBE2T protein in endometrial carcinoma tissues was higher than that in normal endometrial tissues and atypical hyperplasia endometrial tissues.FIGO stage and histological differentiation of endometrial carcinoma tissues showed significant difference in UBE2T expression.Kaplan-Meier survival analysis showed that the average survival time of patients with UBE2T positive expression was shorter than that of patients with negative expression.COX regression analysis showed that FIGO stage,lymph node metastasis and UBE2T expression were prognostic factors of patients with endometrial carcinoma,and FIGO stage and UBE2T expression were independent prognostic factors of endometrial carcinoma.Conclusion The increased expression level of UBE2T in endometrial cancer tissues is closely related to the occurrence,development and poor prognosis of endometrial cancer,and may become an adjunct therapy for endometrial cancer.