多囊卵巢综合征患者血清circPUM1和miR-760的表达变化和临床意义

Expression changes and clinical significance of serum circPUM1 and miR-760 in patients with polycystic ovary syndrome

目的探讨环状RNA PUM1(circPUM1)、微小RNA-760(miR-760)在多囊卵巢综合征(polycystic ovary syndrome,PCOS)患者血清表达情况,及其与患者BMI、生化指标的相关性。方法选择2019年2月至2022年2月在郑州大学附属郑州中心医院诊治的118例PCOS患者做为研究对象(PCOS组),另选同期在本院体检120例女性作为对照组,采用实时荧光定量PCR法(RT-qPCR)检测血清circPUM1、miR-760表达水平,并检测血清各糖脂代谢指标和性激素指标;Pearson法分析circPUM1、miR-760表达水平的相关性,及circPUM1、miR-760与各指标的相关性。结果对照组体质指数(body mass index,BMI)、空腹血糖(fasting blood glucose,FBG)、空腹胰岛素(fasting insulin,FINS)、胰岛素抵抗指数(homeostasis model assessment of insulin resistance index,HOMA-IR)、甘油三酯(triglyceride,TG)、雌二醇、促黄体生成素(luteinizing hormone,LH)、催乳素及circPUM1表达水平分别为(23.81±2.85)kg/m^(2)、(4.87±0.73)mmol/L、(8.51±2.18)mIU/L、1.85±0.69、(1.58±0.57)mmol/L、(182.34±30.47)pmol/L、(7.43±2.05)mIU/ml、(323.45±60.25)mIU/L、1.05±0.21,PCOS组患者分别为(25.24±2.36)kg/m^(2)、(5.35±0.65)mmol/L、(14.43±4.36)mIU/L、3.21±1.05、(1.93±0.60)mmol/L、(193.75±38.42)pmol/L、(10.42±2.60)mIU/mL、(372.31±75.44)mIU/L及1.83±0.45,PCOS组表达水平显著升高;对照组miR-760表达水平为1.01±0.23,PCOS组为0.77±0.16,PCOS组水平显著降低(P<0.05)。相关性分析显示,PCOS患者血清circPUM1与miR-760呈负相关(r=-677,P<0.001),PCOS患者血清circPUM1与FBG、FINS、HOMA-IR、TG呈正相关(P<0.05),与其他指标无明显的相关性(P>0.05);miR-760与FBG、FINS、HOMA-IR、TG呈负相关(P<0.05),与其他指标无明显的相关性(P>0.05)。结论PCOS患者血清circPUM1表达水平升高,miR-760表达水平降低,与糖脂代谢指标相关,可用于预测PCOS患者代谢性疾病发生。

Objective To investigate the expression of serum circular RNA PUM1(circPUM1)and microRNA-760(miR-760)in patients with polycystic ovary syndrome(PCOS),and their correlation with BMI and biochemical indexes.Methods A total of 118 patients with PCOS who were diagnosed and treated in Zhengzhou Central Hospital Affiliated to Zhengzhou University from Feb.2019 to Feb.2022 were regarded as as the research subjects(PCOS group).Another 120 women who underwent physical examination in our hospital were regarded as the control group.Real-time quantitative PCR(RT-qPCR)was performed to detect the expression levels of circPUM1 and miR-760 in serum,and to measure serum glucose and lipid metabolism indicators and sex hormone indicators;Pearson method was performed to analyze the correlation between the expression levels of circPUM1 and miR-760,and the correlation between circPUM1 and miR-760 and each index.Results The expression level of BMI,FBG,FINS,HOMA-IR,TG,estradiol,LH,prolactin and circPUM1 was[(23.81±2.85)kg/m^(2),(4.87±0.73)]mmol/L,(8.51±2.18)mIU/L,1.85±0.69,(1.58±0.57)mmol/L,(182.34±30.47)pmol/L,(7.43±2.05)mIU/ml,(323.45±60.25)mIU/L and 1.05±0.21,while they were(25.24±2.36)kg/m^(2),(5.35±0.65)mmol/L,(14.43±4.36)mIU/L,3.21±1.05,(1.93±0.60)mmol/L,(193.75±38.42)pmol/L,(10.42±2.60)mIU/mL,(372.31±75.44)mIU/L and 1.83±0.45.The expression level of miR-760 was 1.01±0.23 in the control group,while it was 0.77±0.16 in PCOS group.Correlation analysis showed that serum circPUM1 in PCOS patients was negatively correlated with miR-760(r=-677,P<0.001),and serum circPUM1 in PCOS patients was positively correlated with FBG,FINS,HOMA-IR,and TG(P<0.05),while was no greatly correlated with other indicators(P>0.05),and miR-760 was negatively correlated with FBG,FINS,HOMA-IR,and TG(P<0.05),while was no greatly correlated with other indicators(P>0.05).Conclusion The expression level of circPUM1 in serum of patients with PCOS is increased,and the expression level of miR-760 is decreased,which is correlated with glucose and lipid metabolism indicators and can be used to predict the occurrence of metabolic diseases in PCOS patients.