绝经后女性非酒精性脂肪肝患者miR-122-5p及FOXO3水平与骨质疏松的相关性研究

Correlation between serum miR-122-5p and FOXO3 levels and osteoporosis in postmenopausal women with non-alcoholic fatty liver disease

目的探讨绝经后女性非酒精性脂肪肝(non-alcoholic fatty liver disease,NAFLD)患者miR-122-5p及FOXO3为叉头框蛋白O3(forkhead box protein O3,FOXO3)水平与骨质疏松(osteoporosis,OP)的相关性研究。方法收集30例绝经后女性NAFLD患者与48例绝经后女性非NAFLD患者的临床资料及血清。qRT-PCR检测血清中miR-122-5p、FOXO3水平。生化自动分析仪检测甘油三酯、高密度脂蛋白、低密度脂蛋白。骨密度分析仪检测第1~4腰椎、Wards三角骨、股骨颈、大转子、全髋5个部位的骨密度。分析以上临床指标与骨质疏松的相关性。结果miR-122-5p在绝经后女性NAFLD患者中表达为0.76±0.28,较非NAFLD患者的1.00±0.31表达低(t=3.43,P=0.001)。借助生物信息学网站分析得出miR-122-5p的下游靶基因FOXO3。FOXO3在绝经后女性NAFLD患者中表达为1.31±0.30,较非NAFLD患者的1.00±0.27表达升高(t=4.73,P<0.001)。Student’s t检验与Logistic回归分析显示甘油三酯、miR-122-5p、FOXO3水平是NAFLD患病的危险因素(均P<0.05)。Pearson相关系数显示miR-122-5p水平与股骨颈(r=0.49,P=0.006)、大转子(r=0.37,P=0.046)、全髋(r=0.40,P=0.027)的骨密度呈显著正相关,FOXO3水平与股骨颈(r=-0.45,P=0.014)、全髋(r=-0.51,P=0.004)骨密度呈显著负相关,其余指标相关性不明显(均P>0.05)。结论绝经后女性NAFLD患者血清miR-122-5p水平降低、FOXO3水平升高可能增加患者患骨质疏松的风险。

Objective To investigate the correlation between serum miR-122-5p and FOXO3 levels and osteoporosis(OP)in postmenopausal women with non-alcoholic fatty liver disease(NAFLD).Methods The clinical data and serum of 30 postmenopausal women with NAFLD and 48 postmenopausal women with no-NAFLD were collected.The levels of miR-122-5p and FOXO3 in serum were detected by qRT-PCR.Triglycerides,high-density lipoproteins,and low-density lipoproteins were detected by biochemical autoanalyzer.The bone mineral density of lumbar vertebrae 1-4,Wards triangular bone,femoral neck,greater trochanter and total hip was detected by bone mineral density analyzer.The correlation between the above clinical indicators and OP was analyzed.Results The expression of miR-122-5p in postmenopausal female NAFLD patients(0.76±0.28)was lower than that in non-NAFLD patients(1±0.31)(t=3.43,P=0.001).The downstream target gene FOXO3 of miR-122-5p was identified by bioinformatics website analysis.The expression of FOXO3 in postmenopausal female NAFLD patients(1.31±0.30)was higher than that in non-NAFLD patients(1±0.27)(t=4.73,P<0.001).Student’s t test and Logistic regression analysis showed that triglyceride,miR-122-5p and FOXO3 levels were risk factors for NAFLD(all P<0.05).Pearson correlation coefficient showed that miR-122-5p level was significantly positively correlated with BMD of femoral neck(r=0.488,P=0.006),greater trochanter(r=0.367,P=0.046)and whole hip(r=0.404,P=0.027).FOXO3 level was negatively correlated with bone mineral density of femoral neck(r=-0.445,P=0.014)and whole hip(r=-0.507,P=0.004),while other indexes were not significantly correlated(all P>0.05).Conclusion Decreased serum miR-122-5p level and increased FOXO3 level in postmenopausal women with NAFLD may increase the risk of OP.