中频治疗技术增加家兔背阔肌容积的实验研究

Medium-frequency electrotherapy in increasing the volume of latissimus dorsi muscle muscle in rabbits

目的探讨中频治疗技术对家兔背阔肌容积增加的作用。方法2022年4—10月,于清华大学动物实验中心开展。15只成年新西兰白兔分为A、B、C组,每组5只。右侧背阔肌为实验侧,左侧背阔肌为对照侧。分别对应A组6档(7.062 mA)、B组9档(10.593 mA)、C组12档(14.124 mA)3个不同档位的电流强度。分别于实验12、24、36次后用超声采集背阔肌肌肉厚度数据,并于电刺激36次结束后,取背阔肌标本测量活体肌肉厚度、湿重,分别送HE染色、MASSON染色。结果A、B组在电刺激12、24、36次后超声采样显示,右侧背阔肌厚度均较左侧增加,B组右侧较左侧厚度增加37.89%。A、B组电刺激36次后采集的湿重数据显示,右侧背阔肌湿重均较左侧增加,B组右侧较左侧肌肉质量增加5.04%。结论中频治疗技术的不同电刺激模式可引发肌纤维增粗或萎缩。中频治疗技术的9档(10.593 mA)可成为诱导肌纤维增粗的适宜选择,12档(14.124 mA)可成为诱导骨骼肌变纤细的适宜选择。

Objective To explore the safety and effectiveness of medium-frequency electrotherapy for increasing the volume of the latissimus dorsi muscle.Methods Fifteen adult New Zealand white rabbits were randomly divided into three groups,namely group A,group B,and group C,with 5 rabbits in each group.This was a self-control study,with the right latissimus dorsi muscle as the experimental group and the left latissimus dorsi muscle as the control group.The three groups corresponded to three different current intensity levels:7.062 mA for group A(6th gear),10.593 mA for group B(9th gear),and 14.124 mA for group C(12th gear).After the 12th,24th,and 36th sessions of the experiment,ultrasonography was used to collect the thickness of the latissimus dorsi muscle.After the 36th electrostimulation,the latissimus dorsi muscle samples were collected to measure their in vivo muscle thickness and wet weight and were then sent for HE and MASSON staining.Results After the 12th,24th,and 36th electrostimulation sessions,ultrasonographic sampling in groups A and B showed an increase in the thickness of the right latissimus dorsi muscle compared to the left;for example,the thickness on the right of group B increased by 37.8%.The wet weight data collected after the 36th electrostimulation in groups A and B showed an increase in the right latissimus dorsi muscle compared to the left;for example,the wet weight on the right of group B increased by 5.04%.Conclusions Different electrostimulation modes of medium-frequency therapy technology can induce muscle fiber thickening or atrophy.In this experiment,the 9th gear(10.593 mA)of medium-frequency therapy technology may be a suitable choice for inducing muscle fiber thickening,and the 12th gear(14.124 mA)may be a suitable choice for inducing skeletal muscle thinning.