摘要
木聚糖是自然界中含量第二丰富的多糖,是一种可再生资源,木聚糖酶能够降解木聚糖,被广泛应用于化工、食品、饲料、造纸、废物处理和制药等工业领域。大多数天然木聚糖酶热稳定性较差,利用基因工程和蛋白质工程手段对木聚糖酶进行改造,使木聚糖酶热稳定性提高,对工业生产有着重要的现实意义。在分析耐热木聚糖酶结构特点的基础上,对提高木聚糖酶热稳定性的技术手段的研究进展进行了综述,包括固定化、定向进化、半理性设计、理性设计、构建嵌合体,为拓宽耐热木聚糖酶的应用范围提供了帮助。
Xylan is the second abundant polysaccharide in nature,and it is a renewable resource.Xylanase can degrade xylan,thus it is widely used in the industrial fields of chemical industry,food,feed,paper,waste treatment,and pharmaceuticals.Most natural xylanases have poor thermostability.Various genetic engineering and protein engineering means are used to transform xylanase and improve the thermostability of xylanase,which has important practical significance in industrial production.In this review,on the basis of the analysis of the structural characteristics of thermostable xylanase,we summarize the research progress of the technical means to improve the thermostability of xylanase,including immobilization,directional evolution,semi-rational design,rational design,and chimera construction,which provides assistance in expanding the application fields of thermostable xylanase.
作者
李曦月
倪孟祥
LI Xiyue;NI Mengxiang(China Pharmaceutical University,Nanjing 210000,China)
出处
《化学与生物工程》
CAS
2023年第12期1-5,共5页
Chemistry & Bioengineering
基金
国家重点研发计划项目(2019YFA090404)。
关键词
耐热木聚糖酶
结构特点
热稳定性
基因工程
蛋白质工程
thermostable xylanase
structural characteristic
thermostability
genetic engineering
protein engineering
