lncRNA 00628通过TOP2A影响肺腺癌细胞顺铂敏感性

Long non-coding RNA 00628 affects cisplatin sensitivity of lung adenocarcinoma cells by TOP2A

目的:研究lncRNA 00628对肺腺癌细胞顺铂敏感性的影响及可能分子机制。方法:通过数据库筛选肺腺癌组织和癌旁组织中差异表达的lncRNA;运用CCK-8、克隆形成、Transwell和流式细胞仪探究肺腺癌细胞的生物学功能,体内外实验研究linc00628对肺腺癌细胞顺铂敏感性的影响;RNA测序筛选出linc00628的下游靶基因TOP2A,并通过qRT-PCR和Western blot验证;体外实验探究TOP2A对肺腺癌细胞顺铂敏感性的影响。结果:linc00628在肺腺癌组织和细胞中显著高表达;下调linc00628可在体内外增强肺腺癌细胞的顺铂敏感性;TOP2A是linc00628的下游靶基因;在体外,下调TOP2A可增强肺腺癌细胞的顺铂敏感性。结论:linc00628在肺腺癌组织和细胞中高表达,下调linc00628可能通过抑制TOP2A的表达来增强肺腺癌细胞顺铂敏感性。

Objective:To investigate the effect of long non-coding RNA(lncRNA)00628 on cisplatin sensitivity of lung adenocarcinoma cells and its possible molecular mechanism.Methods:The different expression of lncRNAs in lung adenocarcinoma tissues and adjacent normal tissues were screened by database.CCK-8,clone formation,Transwell and flow cytometry were used to investigate the biological function of lung adenocarcinoma cells,and the effect of linc00628 on cisplatin sensitivity of lung adenocarcinoma cells was studied in vitro and vivo test.TOP2A,the downstream target gene of linc00628,was screened by RNA sequencing and verified by qRT-PCR and Western blot.The effect of TOP2A on cisplatin sensitivity of lung adenocarcinoma cells was investigated in vitro.Results:linc00628 was highly expressed in lung adenocarcinoma tissues and cells.Down-regulating linc00628 can enhance cisplatin sensitivity of lung adenocarcinoma cells in vitro and in vivo.TOP2A was the downstream target gene of linc00628.In vitro,downregulation of TOP2A enhanced cisplatin sensitivity in lung adenocarcinoma cells.Conclusion:linc00628 is highly expressed in lung adenocarcinoma tissues and cells.Down-regulation of linc00628 may enhance cisplatin sensitivity of lung adenocarcinoma cells by inhibiting TOP2A expression.