基于荧光光度法的水中石油类污染物便携原位测定装置研制与应用

Development and Application of Portable In-situ Device for Determination of Petroleum Contaminants in Water Based on Fluorescence Photometry

研制了一种基于荧光光度法的水中石油类污染物原位测定装置,该装置采用浸入式水下探头设计,通过测定石油类污染物中芳烃组分的荧光强度检测水中石油类污染物。实验考察了最佳激发波长与发射波长以及不同激发光源对测定结果的影响,设计开发了适于水下检测的一体式光路。结果表明,研制的原位测油装置在水中油质量浓度在0~20 mg/L范围时具有良好线性结果;在1 mg/L和10 mg/L下仪器的示值误差小于3%;在10 mg/L时,重复性相对标准偏差(RSD)为2.6%;研制装置的原位检测结果与实验室萃取-荧光检测结果的一致性良好(R=0.9998),实际样品的加标回收率为91.2%~114%。该装置无需使用有机溶剂萃取,具有良好的准确性与稳定性,可应用于水中油的快速、原位检测。

An in-situ device based on fluorescence photometry for the determination of petroleum contaminants in water is developed.This device is designed as an immersion underwater probe to measure the concentration of petroleum pollutants in water based on the fluorescence intensity of aro⁃matic components in petroleum pollutants.The influence of optimal excitation wavelength and emis⁃sion wavelength and different excitation light sources on determination are investigated,and an inte⁃grated underwater detection optical path is designed.The results show that the in-situ oil measuring device has a good linear results(r^(2)=0.9991)in the concentration range of 0-20 mg/L oil in water.And the indication error of device is within 3%at concentration of 1 mg/L and 10 mg/L,the repeat⁃ability relative standard deviation(RSD)is 2.6%at concentration of 10 mg/L.The result determined by in-situ oil measurement device is highly consistent with laboratory measurement(n-hexane extrac⁃tion-fluorescence detection)(R=0.9998).The recoveries of real samples are between 91.2%and 114%.This device does not require the use of organic solvent extraction and has good accuracy and stability,which can be applied to the rapid and in-situ detection of oil in water.