基于高分辨质谱的食品致敏蛋白检测研究进展

Research Progress on Detection of Food Allergic Protein Based on High-resolution Mass Spectrometry

食品致敏原事关食品安全,其检测技术的进步对于食物过敏风险评估具有重要现实价值。与传统的酶联免疫吸附(ELISA)等免疫学检测方法相比,高分辨质谱技术(HRMS)凭借高通量等特点,近些年在食品致敏蛋白检测研究中的应用日渐广泛。该文主要综述了基于HRMS技术的致敏蛋白检测研究进展,重点介绍了致敏蛋白的提取纯化、酶解以及基于HRMS的分析策略和数据采集模式,同时分析了HRMS在致敏蛋白检测方面存在的挑战,以期为促进致敏蛋白检测的现代化发展提供参考。

Food allergen is tightly related to food safety,and technological progresses on the issue are realistically valuable to food allergy risk assessment.Compared with conventional immunological technique such as enzyme-linked immunosorbent assay(ELISA),high-resolution mass spectrometry(HRMS)is burgeoning in recent years for food allergic protein with its high-throughput characteriza⁃tion.In this paper,the research progress of HRMS technology for allergic protein detection is mainly reviewed,and extraction,purification and enzymatic hydrolysis of allergic protein,as well as HRMS-based analysis strategy and data acquisition is emphatically highlighted.Additionally,the challenges in the detection of allergic protein were also analyzed.