荔枝核香薰疗法对慢性不可预见性应激抑郁模型大鼠海马及血清5-HT、DA的干预作用

Intervention effect of lychee seed aromatherapy on hippocampus and serum 5-HT and DA of chronic unpredictable mild stress depression model rats

目的探讨荔枝核香薰疗法对慢性不可预见性应激(CUMS)抑郁模型大鼠海马及血清5-羟色胺(5-HT)、多巴胺(DA)的干预作用。方法以孤养结合CUMS抑郁模型大鼠作为研究对象,在实验第7、14、21、28天记录每组大鼠的体重增长数、糖水消耗量,在实验第35天治疗结束后,检测各组大鼠海马及血清中兴奋性神经递质5-HT、DA水平。结果(1)体重增长:第14~21天时,与空白组比较,模型组、百忧解组、百忧解辅香薰疗法组大鼠体重增长数均减少(P<0.01)。第28~35天,与空白组比较,模型组大鼠体重增长数减少(P>0.05);与模型组比较,百忧解组、百忧解辅香薰疗法组大鼠体重增长数均增加(P>0.05);与百忧解组比较,百忧解辅香薰疗法组大鼠体重增长数增加(P<0.01)。(2)糖水消耗量变化:第14、21天,与空白组比较,模型组、百忧解组、百忧解辅香薰疗法组大鼠糖水消耗量均减少(P<0.01)。第35天,与空白组比较,模型组大鼠糖水消耗量减少(P<0.01),百忧解组大鼠糖水消耗量减少(P<0.05),百忧解辅香薰疗法组大鼠糖水消耗量减少(P>0.05);与模型组比较,百忧解组大鼠糖水消耗量增加(P<0.05),百忧解辅香薰疗法组大鼠糖水消耗量增加(P<0.01);与百忧解组比较,百忧解辅香薰疗法组大鼠糖水消耗量均增加(P<0.05)。(3)HT、DA水平:与空白组比较,模型组、百忧解组大鼠5-HT、DA水平均明显减少(P<0.01),百忧解辅香薰疗法组大鼠5-HT、DA水平均减少(P>0.05);与模型组比较,空白组、百忧解组、百忧解辅香薰疗法组大鼠5-HT、DA水平均明显升高(P<0.01);与百忧解组比较,百忧解辅香薰疗法组大鼠5-HT、DA水平均明显升高(P<0.05)。结论荔枝核香薰辅助治疗可增加抑郁模型大鼠内单胺类神经递质含量,荔枝核香薰的抗抑郁机制可能与增加脑内单胺类神经递质有关。

Objective To explore the intervention effect of lychee seed aromatherapy on hippocampus and serum 5-hydroxytryptamine(5-HT)and dopamine(DA)in chronic unpredictable mild stress(CUMS)depression model rats.Methods The isolatedfeed model rats withchronic unpredictable mild stress were used as the research object,the body weight gain and sugar and water consumption of rats in each group were recorded and calculated on the 7th,14th,21st and 28th days of the experiment.The contents of excitatory neurotransmitters 5-HT and DA in hippocampus and serum of rats in each group were detected after treatment on the 35th day of the experiment.Results(1)Weight gain:From 14th to 21st day,compared with the blank group,the weight gain of rats in the model group,the prozac group and the prozac supplemented aromatherapy group was decreased(P<0.01)at the 14th to 21st day;From the 28th to the 35th day,compared with the blank group,the weight gain of rats in the model group decreased(P>0.05),compared with the model group,the weight gain of rats in the prozac group and the prozac supplemented aromatherapy group increased(P>0.05),compared with the prozac supplemented aromatherapy group,the weight gain of rats in the prozac group increased(P>0.05).The number of body weight gain of rats in the prozac supplemented aromatherapy group was increased(P<0.01).(2)Changes in sugar water consumption:on the 14th and 21st day,compared with the blank group,rats in the model group,the prozac group and the prozac supplemented aromatherapy group all decreased(P<0.01);On the 35th day,compared with the blank group,the number of rats in the model group decreased(P<0.01),the number of rats in the prozac group decreased(P<0.05),the number of rats in the prozac group supplemented with aromatherapy decreased(P>0.05),and the number of rats in the prozac group increased(P<0.05),while the number of rats in the model group increased(P<0.05).The number of rats in the prozacsupplemented aromatherapy group increased(P<0.01);Compared with the prozac group,the number of rats in the prozac supplemented aromatherapy group was increased(P<0.05).(3)Compared with the blank group,5-HT and DA concentrations in the model group and the prozac group were significantly decreased(P<0.01),and 5-HT and DA concentrations in the prozac supplemented aromatherapy group were significantly decreased(P>0.05).Compared with the model group,the concentrations of 5-HT and DA in the blank group,the prozac group and the prozac supplemented aromatherapy group were significantly increased(P<0.01).Compared with the prozac group,the concentrations of 5-HT and DA in the prozac supplemented aromatherapy group were significantly increased(P<0.05).Conclusion Lychee seed aromatherapy can increase the content of monoamine neurotransmitters in the depressed model rats.The anti-depression mechanism of lychee seed aromatherapy may be related to the increase of monoamine neurotransmitters in the brain.