放射治疗对宫颈癌DNA甲基转移酶表达的影响

Effect of radiotherapy on the expression of DNA methyltransferase in cervical cancer

目的:研究放射治疗对宫颈癌(cervical cancer,CIN)组织中DNA甲基转移酶(DNA methyltransferase,DNMT)1、3A和3B表达的影响,探讨其在放射治疗耐受中的作用。方法:选用12例宫颈癌患者放射治疗前、中、后三个时期的组织,应用qPCR检测宫颈癌组织在放射治疗前期、中期和后期的DNMT1、DNMT3A和DNMT3B mRNA的表达变化。结果:宫颈癌放射治疗前、中、后三个时期DNMT1对β-actin mRNA的相对表达量分别是(0.1229±0.0217)、(0.5696±0.0217)和(0.1620±0.0352);放射治疗期间和放射治疗之前宫颈癌组织中DNMT1 mRNA的表达差异有统计学意义(P<0.05),放射治疗期间和放射治疗之后宫颈癌组织中DNMT1 mRNA的表达差异有统计学意义(P<0.05),放射治疗之前和放射治疗之后宫颈癌组织中DNMT1 mRNA的表达差异有统计学意义(P<0.05);DNMT3A对β-actin mRNA的相对表达量分别是(0.0012±0.0001)、(0.0021±0.0001)和(0.0014±0.0001),放射治疗期间和放射治疗之前宫颈癌组织中DNMT3A mRNA的表达差异有统计学意义(P<0.05),放射治疗期间和放射治疗之后宫颈癌组织中DNMT3A mRNA的表达差异有统计学意义(P<0.05),放射治疗之前和放射治疗之后宫颈癌组织中DNMT3A mRNA的表达差异无统计学意义(P>0.05);DNMT3B对β-actin mRNA的相对表达量分别是(0.6627±0.0348)、(0.8104±0.0845)和(0.2391±0.0470),放射治疗期间和放射治疗之前宫颈癌组织中DNMT3B mRNA的表达差异有统计学意义(P<0.05),放射治疗期间和放射治疗之后宫颈癌组织中DNMT3B mRNA的表达差异有统计学意义(P<0.05),放射治疗之前和放射治疗之后宫颈癌组织中DNMT3B mRNA的表达差异有统计学意义(P<0.05)。结论:放射治疗使宫颈癌组织中DNMT1,DNMT3A和DNMT3B表达发生变化,其可能在放射耐受中起到了作用。

Objective:To investigate the effect of radiotherapy on the expression of DNA methyltransferase(DNMT)1,3A,and 3B in cervical cancer(CIN)tissues,and to explore its role in radiotherapy resistance.Methods:The cervical cancer tissues of 12 patients were selected before,during and after radiotherapy.The mRNA expression levels of DNMT1,DNMT3A and DNMT3B in cervical cancer tissues after radiotherapy were detected by qPCR.Results:The relative expression levels of DNMT1 toβ-ACTIN were 0.1229±0.0217,0.5696±0.0217 and 0.1620±0.0352 respectively before,during and after radiotherapy for cervical cancer.The expression of DNMT1 mRNA in cervical cancer tissues during and before radiotherapy was significantly different(P<0.05),and the expression of DNMT1 mRNA in cervical cancer tissues during and after radiotherapy was significantly different(P<0.05).The expression of DNMT1 mRNA in cervical cancer tissues before and after radiotherapy was significantly different(P<0.05).The relative expression levels of DNMT3A toβ-ACTIN mRNA were 0.0012±0.0001,0.0021±0.0001 and 0.0014±0.0001 respectively.There were significant differences in DNMT3A mRNA expression in cervical cancer tissues during radiotherapy and before radiotherapy(P<0.05).The expression of DNMT3A mRNA in cervical cancer tissues during and after radiotherapy was significantly different(P<0.05),however,the expression of DNMT3A mRNA before and after radiotherapy was not significantly different(P>0.05).The relative expression of DNMT3B onβ-ACTIN mRNA was 0.6627±0.0348,0.8104±0.0845 and 0.2391±0.0470 respectively.The mRNA expression of DNMT3B was significantly different in cervical cancer tissues during and before radiotherapy(P<0.05).The expression of DNMT3B mRNA in cervical cancer tissues during and after radiotherapy was significantly different(P<0.05),and the expression of DNMT3B mRNA in cervical cancer tissues before and after radiotherapy was significantly different(P<0.05).Conclusion:Radiotherapy changes the expression of DNMT1,DNMT3A and DNMT3B in cervical cancer tissues,which may play an important role in radioresistance.