微小RNA-155和血清β2微球蛋白检测在血液透析患者并发心血管疾病中的诊断价值

Diagnostic value of microRNA-155 and serumβ2-microglobulin in hemodialysis patients with cardiovascular disease

目的:探究血液透析患者中微小RNA-155(miRNA-155)和血清β2微球蛋白的表达与心血管疾病风险的相关性。方法:选择100例血液透析患者中患有心血管疾病的患者为试验组,100例血液透析患者中无心血管疾病的患者为对照组。采取血液透析患者一定量的静脉血样,检测患者血清中可溶性生长刺激表达基因2蛋白(sST2)、肌钙蛋白T(TNT)与血清氨基末端脑利钠肽前体(NT-proBNP)。对所有研究对象的心血管疾病进行评估。采用实时荧光定量PCR(qPCR)检测血清中的miRNA-155的水平,采用速率散射比浊法检测血清β2微球蛋白水平。结果:试验组的年龄、糖尿病史的比例、开始透析的平均年龄、TNT平均水平、NT-proBNP平均水平与sST2平均水平高于对照组(均P<0.05)。同时,对照组的平均透析时间要高于试验组(P<0.05)。试验组血清中的miRNA-155含量明显低于对照组血清中的miRNA-155的含量(P<0.05),同时血清miRNA-155表达水平与TNT、NT-proBNP、sST2的水平呈现负相关性(均P<0.05)。试验组血清中的β2微球蛋白含量明显高于对照组血清中的β2微球蛋白的含量(P<0.05),同时血清β2微球蛋白水平与TNT、NT-proBNP、sST2的水平呈现正相关性(均P<0.05)。ROC曲线分析显示血清miRNA-155与β2微球蛋白诊断的曲线下面积(AUC)分别为0.912(95%CI:0.798~0.985)与0.842(95%CI:0.793~0.972)。结论:sST2、TNTNT-proBNP可以有效地对血液透析患者的心血管疾病作出诊断,同时患有心血管疾病的血液透析患者血清中miRNA-155表达水平较低,血清β2微球蛋白的表达水平较高;因此miRNA-155与血清β2微球蛋白在预测血液透析患者并发心血管疾病时具有较高价值。

Objective:To investigate the correlation between the expression of microRNA-155(miRNA-155),serumβ2-microglobulin and the risk of cardiovascular disease in hemodialysis patients.Methods:A totalo of 100 hemodialysis patients which have cardiovascular diseases were chose as the experimental group.At the same time,100 hemodialysis patients without cardiovascular were selected as the control group.There were blood samples collection from the 200 selected hemodialysis patients,and the serum soluble growth stimulating gene 2 protein(sST2),troponin T(TNT)and serum N-terminal pro-brain natriuretic peptide(NT-proBNP)were detected.All subjects were assessed for cardiovascular disease.The level of miRNA-155 in serum was examined by qPCR.The serumβ2-microglobulin level was detected by rate scattering turbidimetry.Results:The age,the proportion of diabetes history,the average age of starting dialysis,the average level of TNT,the mean level of NT-proBNP and sST2 in the experimental group were higher than those in the control group(all P<0.05).At the same time,the average dialysis time in the control group was longer than the average dialysis time in the experimental group(P<0.05).The content of miRNA-155 in serum of experimental group was significantly lower than that of control group(P<0.05).Meanwhile,the expression level of serum miRNA-155 was negatively correlated with the levels of TNT,NT-proBNP and sST2(all P<0.05).The content ofβ2-microglobulin in serum of experimental group was significantly higher than that of control group(P<0.05).At the same time,the level of serumβ2-microglobulin was positively correlated with the levels of TNT,NT-proBNP and sST2(all P<0.05).ROC curve analysis showed that the AUC of serum miRNA-155 andβ2-microglobulin were 0.912(95%CI:0.798 to 0.985)and 0.842(95%CI:0.793 to 0.972),respectively.Conclusion:The sST2,TNT and NT-proBNP can effectively diagnose cardiovascular diseases in hemodialysis patients,and the expression level of miRNA-155 in serum of hemodialysis patients with cardiovascular diseases is low,the expression level of serumβ2-microglobulin is high.Therefore,miRNA-155 and serumβ2-microglobulin have outstanding value in predicting cardiovascular diseases in hemodialysis patients.