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大米中T-2毒素质控样品的研制

Preparation of T-2 Toxin Quality Control Samples in Rice
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摘要 目的:采用研磨的方式制备以大米为基质的T-2毒素质控样品。方法:将不含T-2毒素的大米经研磨过筛、添加标准物质,再次进行研磨混匀,置于无菌采样袋中密封分装。用液相色谱-串联质谱对质控样进行含量检测,参照CNAS-GL003:2018《能力验证样品均匀性和稳定性评价指南》对样品进行均匀性和稳定性分析,采用与6家实验室协作定值的方式确定质控样的参考值。结果:单因素方差分析中,F<F_(0.05),表明制备的T-2毒素质控样品在样品间不存在显著性差异;t检验中,t<t_(0.05),表明样品中的分析物在12个月内是稳定的。最终确定大米中T-2毒素的定值结果为8.79μg·kg^(-1)。结论:该方法制得的质控样品可用于粮食中T-2毒素污染情况质量监控、能力验证、快检产品评价等领域,并为进一步研制粮食中其他毒素质控样提供技术支持。 Objective:To prepare quality control sample of T-2 toxin based on rice by grinding.Method:The rice without T-2 toxin was grinded,sieved,added with T-2 toxin standard materials,grinded again,and then packed in sterile sampling bags.The content of the quality control samples was detected by liquid chromatography-tandem mass spectrometry.According to CNAS-GL003:2018,the uniformity and stability of T-2 toxin quality control samples were evaluated and cooperated with 6 laboratories to definite value.Result:In the one-way analysis of variance,F<F_(0.05),indicating that there was no significant differences among the prepared control samples.In the t-test method,t<t_(0.05),indicating that the stability of the samples during the 12 month storage period was good.Finally,the standard value of T-2 toxin quality control samples was determined 8.79μg·kg^(-1).Conclusion:The quality control samples obtained by this method can be applied to quality monitoring of T-2 toxin contamination in grain,ability verification,product evaluation and other fields,and provide technical support for the further development of the quality control samples of other toxin.
作者 邓皇翼 刘海虹 刘耀慧 罗志浩 蔡若纯 DENG Huangyi;LIU Haihong;LIU Yaohui;LUO Zhihao;CAI Ruochun(Guangdong Institute for Food Inspection,Guangzhou 510435,China)
出处 《食品安全导刊》 2023年第23期72-76,80,共6页 China Food Safety Magazine
关键词 大米基质 T-2毒素 质控样品 均匀性 稳定性 定值 rice matrix T-2 toxin quality control sample uniformity stability fixed value
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