FOXM1和PUM1在结肠癌组织中的表达及其与患者临床病理特征和预后的关系

Expression of FOXM1 and PUM1 in colon cancer and their relationship with clinicopathological characteristics and prognosis

目的探讨PUM1、叉头盒蛋白M1(FOXM1)在结肠癌组织中的表达及其与患者临床病理特征和预后的关系。方法选择2017年1月至2019年6月在湖州市第一人民医院接受手术治疗的135例结肠癌患者作为研究对象,记录其临床资料并随访3年。收集患者的结肠癌组织及癌旁组织,采用免疫组织化学染色法检测组织中FOXM1、PUM1表达情况;采用蛋白质印迹法检测组织中FOXM1、PUM1表达水平;采用Pearson相关性分析探讨结肠癌组织中FOXM1与PUM1表达的相关性;采用Kaplan-Meier法绘制生存曲线分析结肠癌组织中FOXM1、PUM1表达与患者预后的关系;采用多因素Cox回归分析探讨结肠癌患者预后的影响因素。结果结肠癌组织中FOXM1、PUM1阳性表达率均显著高于癌旁组织(82.22%比13.33%,74.07%比16.30%,P均<0.05)。结肠癌组织中FOXM1、PUM1表达水平均显著高于癌旁组织(P均<0.05)。Pearson相关性分析结果显示,结肠癌组织中PUM1与FOXM1表达呈正相关(r=0.514,P<0.05)。结肠癌组织中PUM1、FOXM1表达与有无远处转移、临床分期、分化程度及有无脉管癌栓均有相关性(P均<0.05)。Kaplan-Meier生存曲线分析结果显示,FOXM1、PUM1阳性表达患者的3年累积生存率均显著低于FOXM1、PUM1阴性表达患者(60.36%比79.17%,log-rankχ^(2)=6.216,P=0.013;58.00%比80.00%,log-rankχ^(2)=6.688,P=0.010)。多因素Cox回归分析结果显示,FOXM1、PUM1、远处转移、临床分期及分化程度均是患者预后的独立危险因素(P均<0.05)。结论FOXM1、PUM1在结肠癌组织中表达均显著升高且呈正相关,两者与有无远处转移、临床分期、分化程度及有无脉管癌栓均有相关性,且均是结肠癌患者预后的独立危险因素。

Objective This paper aimed to investigate the expression of PUM1 and forkhead box protein M1(FOXM1)in colon cancer and their relationship with clinicopathological characteristics and prognosis.Methods A total of 135 patients with colon cancer who were operated in the First People's Hospital of Huzhou from January 2017 to June 2019 were selected as the study objects.They were followed up for 3 years,and their clinical data was recorded.The 135 colon cancer tissue samples and adjacent tissue samples were collected,immunohistochemical staining was used to observe the expression of PUM1 and FOXM1 in colon cancer tissues,Western blotting was applied to detect the expression of PUM1 and FOXM1 in tissues,the Pearson correlation analysis method was used to explore the correlation between FOXM1 and PUM1 expression in colon cancer tissues,The Kaplan-Meier method was used to plot the survival curves and analyze the relationship between FOXM1 and PUM1 expression in colon cancer tissue and patient prognosis,and the multivariate Cox regression analysis was used to explore the factors influencing the prognosis of colon cancer patients.Results The positive expression rates of FOXM1 and PUM1 in colon cancer tissues are significantly higher than those in adjacent tissues(82.22%versus 13.33%,74.07%versus 16.30%,P<0.05).The expressions of FOXM1 and PUM1 in colon cancer tissues are higher than those in adjacent tissues(P<0.05).The Pearson correlation analysis results shows a positive correlation between PUM1 and FOXM1 expression in colon cancer tissue(r=0.514,P<0.05).The expression of PUM1 and FOXM1 in colon cancer tissue are correlated with the presence or absence of distant metastasis,clinical staging,differentiation degree,and the presence or absence of vascular cancer thrombus(P<0.05).The Kaplan-Meier survival curve analysis shows that the 3-year cumulative survival rate of FOXM1 and PUM1 positive expression patients was significantly lower than that of FOXM1 and PUM1 negative expression patients(60.36%versus 79.17%,log-rankχ^(2)=6.216,P=0.013;58.00%versus 80.00%,log-rankχ^(2)=6.688,P=0.010).The results of multivariate Cox regression analysis shows that FOXM1,PUM1,distant metastasis,clinical stage,and degree of differentiation are independent risk factors for patient prognosis(P<0.05).Conclusions The expressions of FOXM1 and PUM1 are significantly increased and positively correlated in colon cancer tissue.Both of them are related to the presence or absence of distant metastasis,clinical staging,differentiation degree,and presence or absence of vascular cancer thrombus,and are independent risk factors for the prognosis of colon cancer patients.