人参多糖分散片制备工艺及其免疫活性研究

Study on Preparation Processes and Immune Activity of Ginseng Polysaccharide Dispersible Tablets

旨在确定人参多糖分散片的处方、制备工艺并检测免疫活性。采用单因素实验法对崩解剂、填充剂和润滑剂进行考察,以性状、崩解时限、硬度为考察指标,优化人参多糖分散片的处方;通过对小鼠脏器指数、迟发性变态反应、吞噬指数和自然杀伤细胞活性的测定考察人参多糖分散片的免疫活性。结果表明,人参多糖分散片的优选处方为31.25%人参粗多糖作主药,38.67%微晶纤维素与19.08%α-乳糖作复合填充剂,10%交联聚乙烯吡咯烷酮作崩解剂,1%硬脂酸镁作润滑剂,50%乙醇作润湿剂。免疫活性试验结果表明,人参多糖分散片可以显著提高小鼠的脏器指数、足跖肿胀程度、吞噬指数和自然杀伤细胞活性,具有良好的免疫增强活性。其中2.50 g/(kg·bw)人参多糖分散片的免疫活性最强,可作为潜在的保健食品或营养补充剂。

The prescription,preparation technology and immune activity of ginseng polysaccharide dispersible tablets were determined in the study.The disintegrants,fillers and lubricants were examined using single factor experiment method.The traits,disintegration time and hardness were used to optimize the prescription of ginseng polysaccharide dispersible tablets.The organ index,delayed hypersensitivity,phagocytic index and natural killer cell activity in mice were determined to examine the immunological activity of ginseng polysaccharide dispersible tablets.The results showed that the optimum prescription of ginseng polysaccharide dispersible tablets was 31.25%ginseng crude polysaccharide as main ingredient,38.67%microcrystalline cellulose and 19.08%α-lactose as composite filler,10%cross-linked polyvinylpyrrolidone as disintegrant,1%magnesium stearate as lubricant and 50%ethanol as wetting agent.The results of immunoactivity test showed that ginseng polysaccharide dispersible tablets could significantly increase organ index,foot-plantar swelling degree,phagocytic index and natural killer cell activity in mice,exhibiting good immune enhancement function.Among them,2.50 g/(kg·bw)ginseng polysaccharide dispersible tablets showed the strongest immunological activity and can be used as potential health food or nutritional supplements.